Study Of Risk Factors And Apolipoprotein E And Apolipoprotein B Polymorphisms In Han And Uygur Populations With Gallstone

Objective:(1) To explore the prevalence rate of gallstone and the risk factors ofgallstone in Han and Uygur population in One Center;(2) to investigate the relationshipbetween ApoE gene tagging SNPs polymorphism and gallstones;(3) to investigate therelationship between ApoB gene tagging SNPs polymorphism and gallstones. Methods:(1)3288cases in one hospital Health Care Center were used to analyze the prevalencerates of gallstones in different nationalities, moreover, to explore the risk factors ofgallstones in different ethnic populations (Han and Uygur) and in different gender anddifferent ages by the non-conditional Logistic regression; and also to explore the differentrisk factors in different groups;(2) A case-control study including158Uygur gallstonesand106controls as well as110Han gallstones and106controls was designed to observethe relationship between the polymorphisms of ApoE and gallstones by the restrictionfragment length polymorphism;(3) A case-control study including158Uygur gallstonesand106controls as well as110Han gallstones and106controls was designed to observethe relationship between the polymorphisms of ApoB and gallstones by the SNaPshotSNP genotyping. Results:(1) The prevalence rate of gallstone is different in differentethnics、genders and ages. The prevalence rate of gallstone in Uygur populations issignificantly higher than the Han nationality (30.66%vs13.39%, P＜0.05), and so doesin different ages (34.34%vs11.51%, P＜0.05), also does in different genders (18.14%vs14.61%, P＜0.05). The risk factors of gallstone is partly different in different ages andgenders. Interestingly, the ethnic is the common risk factor. So we furtherly compared therisk factors and general data of gallstone in Han and Uygur, we found that TG is the samerisk factors and there are different risk factors and different general characteristics in different ethnics. In Han groups BMI is risk factors and female and fatty Live is riskfactors in Uygur groups. Aged、female、fatty Live、more BMI、lower TC and higher LDLis common in Uygur groups;(2) In the Uyghur population, significant difference ingenotype frequencies and allele frequencies of tagSNPs rs405509were found in theApoE gene between patients with gallstones and controls P＜0.05). In the Han population,the difference between the genotype frequencies and allele frequencies of tagSNPsrs405509between gallstone patients and controls was significant (P＜0.05), but nosignificant difference in genotype frequencies and allele frequencies was found fortagSNPs rs769450(P＞0.05).(3) In the Uyghur population, no significant difference ingenotype or allele frequencies of SNPs rs676210, rs2854725, rs11676704, rs520354, orrs1367117were found in the ApoB gene between patients with gallstones and controls (P＞0.05). In the Han population, the difference between the genotype frequencies andallele frequencies of SNPs rs11676704and rs520354between gallstone patients andcontrols was significant (P＜0.05). No significant difference was found for SNPsrs676210, rs2854725and rs1367117(P＞0.05). Conclusions:(1) The prevalence ofgallstone is higher in Uygur population and aged groups. The risk factors are different indifferent ethnic and ages, also and genders. Ethnic is an important risk factors ingallstone. The general characterics and risk factors are different in different ethnics;(2)The distribution of ApoE rs769450is found difference between Han and Uygurgroups.The distribution of ApoE rs769450is not found difference between Han andUygur groups.(3) ApoB rs11676704and rs520354polymorphisms may be related to riskof developing gallstone in the Han population, but not related to risk of gallstone in theUygur population.ApoB rs676210、rs2854725、rs1367117polymorphisms may be norelation to risk of gallstone in both Uygur and Han population.