Research On Therapy Of Rheumatoid Arthritis By Bitong Qing Granule

Objective:Rheumatoid Arthritis(RA) is a kind of chronic multisystematically inflammatory autoimmune disease,marked by Arthromeningitis.Because of its high incidence of disease and rate of disability,it has been one of the international tackled problems.Due to its uncertain pathogenic pathogenic cause and pathogenesis,there is no specific and pertinent drug,moreover clinical anti-rheumatic drugs in western medicine have always a lot of side effects.However,Chinese medicine is mainly characterized by bianzheng lunzhi.It has great advantage to preventing the development of disease through multi-layer,multi-loop,multi-method and multi-target way.The research lies on the clinical experience of treating active stage of tutor and mine.To explore the law of syndrome differentiation and treatment on active stage of RA and the mechanism of action of the therapeutic method through animal experiments and clinical trial.Methods:There are four parts as follows.①Establish adjuvant induced Arthritis rats model,observe Bitong Qing granule for its Arthritis evaluation,arthroncus degree and so on the effects of histopathologic change on ankle joint.②EstablishⅡcollagen induced Arthritis of rats model,observe Bitong Qing granule for its Arthritis evaluation,splenocytes proliferation response,sensitized adhesive capacity of T spleniclym phocvtes and so on the effects of synoviocytes cultured supernatant on IL-1β, TNF-αcontained quantity.③The pain threshold and frequency of body writhing were studied across a range of stimulus intensities in thermal(hot plate) and chemical irritant(acetic acid glacial) tests.④The anti-inflammatory effect was tested by ear swollen method and capillary penetration method in mice.Results:①Bitong Qing granule can obviously reduce adjuvant induced arthritis rats local paw swelling degree,histopathologic change on ankle joint,lower arthritis evaluation.②Bitong Qing granule can obviously reduceⅡcollagen induced arthritis of rats and delay the incidence of Arthritis,obviously inhibit the claws swelling and swelling evaluation scores:inhibit the T lymphocyte proliferation:inhibit an sensitized an sensitized adhesive capacity(adhesion) of T splenocytes:lower synoviocytes cultured supernatant IL-1β,TNF-αlevels.③Bitong Qing granule can prolong the pain threshold of mice and inhibit frequency of body writhing.④Bitong Qing granule can inhibite xylene-induced ear swelling and reduce acetic acid-induced permeability of the capillaries.Conclusion:Bitong Qing granule can that is basic prescription of warming meridian to expel cold associated with dispersing heat has good clinical effect on active stage of RA.The mechanism of curing RA may be in connection with the functions of anti-inflammatory,analgesia and immune regulation.