Mechanism Of White Mange Ointment For The Treatment Of Psoriasis

Objective:The aim of this study is to investigate the effects and mechanisms of Baibi ointment for treating Psoriasis.Methods: 5% propranolol emulsion was applied externally on the back of a guinea pig' s ear to produce the psoriasis-like dermatitis model to observe the effects of Baibi ointment on the histopathological changes of the model.Radioimmunoassay was used to observe the levels of cytokines like IL-2,IL—6 and IL-8, and the effects of the ointment to the cytokines. MTT method was used to study the effects of the extracts of the Baibi Formula and the extracts of its main components Huangjing , Kushen , Dahuang ,Huangbai, to the multiplication of HaCaT cells—one of the Malpighian cell line with and without stimulation of TNF-α.Results:Baibi ointment showed some effects on the histopathological changes of the models. It showed that the Baibi ointment Group was 2.70±0.67 and the model group was 6.20±1.42 by Baker score,p<0.05;while compared with the control group-Camptothecine ointment group which was 3.00±0.58,p >0.05.Baibi ointment was effective to the levels of cytokines also.Increased levels of IL-2,IL-6 were showed in the model group,compare with the controlled blank group p<0.05, decreaed levies of IL-2>IL? were showed in the Baibi Ointment group and Camptothecine ointment group, compared with the model group, p < 0.05.Cell culture experiments showed that the extracts of the Baibi Formula and the extracts of its main components Dahuang, Kushen , Huangbai, Huangjing were effective in inhibiting the multiplication of HaCaT cells with or without the stimulation of TNF-αand the relationship between the two was dose dependent.Conclusion:Baibi ointment has therapeutical effects on the histopathological changes of the models of psoriasis-like dermatitis in guinea pigs. The mechanism is probably by inhibiting the multiplication of the Malpighian cell and regulating the cytokines. Thus, Baibi ointment is an effective external preparation to treat psoriasis, and is worthy of deeper reasearch and generalization.