| Objective: Diabetic peripheral neuropathy(DPN)is one of the most common and chronic complication of Diabetes mellitus(DM),which seriously influence on the life quality of diabetic patients, and be the major factor of mutilation of diabetic patients. Finding an effective way to treat DPN is the researching hot spot of internal and external.Despite the research of the mechanism on the DPN have achieved some results, the therapy to DPN is still poor in the clinic. Now,the research of DPN focus on the endocelluar signal transduction , in order to find the common way , and the new therapy method for the prevention and cure of DPN. This experiment approaches the role of the prevention and cure of the different smoothing collaterals Chinese medicine to DPN mice, and its mechanism, through observing the different smoothing collaterals Chinese medicine to DPN mice induced by STZ, the pathomorph change of sciatic nerve and the albumen expression of NF-κB,IκBa,Ikka in the sciatic nerve.Method: 187 male Wistar rats were randomly divided into five groups, including normal-control group , model-control group , XingWeiTongLuo(XWTL) group , TengLeiTongLuo(TLTL) group, CHongLeiTongLuo(CLTL) group, and diabetic model were induced by STZ.①nonfasting plasma glucose and weigh were measured by vena caudalis in the 2th,4th,8th week and before treatment respectively.②the speed of conduction in sciatic nerve was detected in 2th,4th,8th week, and then the rats were killed in 2th,4th,8th week, the sciatic nerve was taken out ,which was observed by Morphometric evaluation under light microscope and transmission electron microscope, and observed NF-κB,IκBa,Ikka albumen express of the sciatic nerve by immunohistochemical method and western blotting method.Results:①There were a significantly difference between normal-control group and model-control group in the level on plasma glucose and weight after making model. There was a raising up tendency in the level of in the plasma glucose, but weight increased slowly in 2th,4th,8th week. compared with model-control group, there were a bit lower in the level of plasma glucose and a bit grower in the weight, but not statistically significant , and there were not a significantly difference on the plasma glucose and weight in the XWTL group, TLTL group, CLTL group.②The effects of different smoothing collaterals Chinese medicine on the sciatic nerve conduction velocity: compared with the normal group, the sciatic nerve conduction velocity of the model group is significantly slow on the 2th,4th,8th week after successful induction by STZ(P<0.01).The different smoothing collaterals Chinese medicine groups could accelerate the nerve conduction velocity .And at the same time there also was a significant difference between the different smoothing collaterals Chinese medicine groups and model group(P<0.01 ).③Observation on pathological change: Compared with normal-control group, there were obvious reactions to the damage all in model group, XWTL group, TLTL group, CLTL group in the 2th,4th,8th week, however the reaction in the different smoothing collaterals Chinese medicine groups is weaker obviously,the damage of the therapeutic groups is similar.④The results of immunohistochemical and western blotting method show: The level NF-κB of sciatic nerve in the making model group is obviously higher than the normal-control group in the corresponding time period (P<0.01),however the level of IκBa,Ikka is lower obviously than the normal-control group in the corresponding time period(P<0.01), and following the prolongation of the disease course, the level NF-κB of sciatic nerve in the model-control group was increased gradually, the level IκBa,Ikka is lower gradually. Compared with the model-control group in the corresponding time period, the different smoothing collaterals Chinese medicine groups in the 4th and 8th week can lower the expression of NF-κB in the sciatic nerve(P<0.01), and elevate the expression of IκBa,Ikka(P<0.01), in the 2th week between the therapeutic groups and model-contrio group has no defference(P>0.05 ). There were no obviously different during the three therapeutic groups(P>0.05 ).Conclusion: The different smoothing collaterals Chinese medicine can lessen the damages to structure and function of peripheral nerves in diabetic rats and postpone the development of neuropathy. The protective effects of the different smoothing collaterals Chinese medicine on diabetic peripheral neuropathy is possibly related with regulating the NF-κB signal transduction system. |
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