Dampness Constitution Mechanism And Gene Expression Profiles

Objective Phlegm-dampness constitution is one of the common types of constitution inmodern society. It is closed related to such diseases such as diabetes, primaryhypertension, coronary heart disease, obesity and apoplexy. It is very helpfulfor screening traditional Chinese drugs in the regulation of abnormalconstitution in clinics to theoretically explore the causes of phlegm-dampnessconstitution. Thus,some diseases related to phlegm-dampness constitution couldbe effectively prevented.To compared and analyzed the gene expression ofperipheral blood profiles among peoples with phlegm-dampness constitutionpersons, obese constitution persons and normal constitution by summarizing theliterature and gene-chip test. To provide the theoretical basis for furtherstudying the characteristics of phlegm-dampness constitution by exploring itsmechanism and observaing its pathogenesis, susceptiblity and the law oftreatment.Methods 3 phlegm-dampness constitution persons(group A), 2 obese constitutionpersons(group B)and 3 normal constitution persons(group C) were selected, totalRNA extracted from peripheral blood was amplified and labelled. Samples weredetected by Affymetrix human genome Test 3 array and gene expression profileswas observed by Affymetrix human genome U133A set. analytic result for each chipwas obtained by GCOSv1.2, meanwhile the data quality was controlled. One wayANOVA was employed, 100 genes with significant differences between every twogroups (a b,a c and b c ) was picked out respectively for 3 times, 300 geneswas obtained. After the Symphyso-collection (278 genes) of the 300 genes wasgot, cluster analysis was done by Cluster software and TreeView. To furtheranalyze the biological function of both the up-regulation and down regulationgenes of phlegmatic hygrosis constitution,20 genes with significant differencesbetween groups (a b,and a c) was picked out,excluding weak signal ,biologicalfunction for genes with ratio>1.5(up-regulated)and ratio<0.67(down regulated)was analyzed.Results Of the original 22283 genes,10308 genes conformed to the standard foranalysis by data filtrating. 168 differential gene were found betweenphlegm-dampness constitution group and non- phlegm-dampness constitutiongroup(obese and normal group). By clustering analysis, group , 3 differentgenetic characters were obtained.Conclusions 1. The the main mechanism of phlegm-dampness constitution was that theessence was unable to transform . The dysfunction of transportation and thedisorder of transformation essence leaded to the formation of the constitution. 2. Phlegm-dampness constitution was the etiology of many diseases. Thecommon pathogenesis of phlegm-dampness constitution was consumption of Yang Qi ,the stagnation of Qi and Phlegm complicated with stasis . Invigorating spleenand dispering essence was the main method for regulating phlegm-dampnessconstitution. 3. Phlegm-dampness constitution has its unique gene expression profile. Oneof the molecular mechanisms of phlegm-dampness constitution was theup-regulation of GNPDA1 and down-regulation of CACNB2. 4. Phlegm-dampness constitution can be regulated , and it should be treatedat stage when one is subhealthy .