Stasis Physical Theory And Its Peripheral Blood Gene Expression Profiles

Objective To excavate the related theories of Stagnant Blood Constitution(SBC) from Traditional Chinese Medicine theory,summarize contemporary research achievement and expatiate the theories of SBC and its application.To reveal the characteristics of gene expression for SBC by detecting the gene expression profiles of peripheral blood of SBC.It will provide theory basis to prevent related diseases early.Methods By the hods of the traditional literature research and the contemporary literature analysis,we discuss the correlative theories of SBC from origin to development,from connotation to extension.13 typical subjects of SBC and 30 subjects of normal constitution were selected on the basis of clinical epidemiological investigation.Ten milliliters of fasting venous blood were collected from each subject.The white blood cells were isolated,and the total RNA was extracted,purified and amplified.The complement RNA(cRNA) was labelled with biotin.The gene expression profiles of peripheral blood were detected with Sentrix Human-6 Expression BeadChip gene chip.The differentially expressed genes as compared with normal constitution were analyzed,and they were GO annotated and statistically pathway analyzed.The biological functions of differentially expressed genes were evaluated in order to reveal the cause,characteristics and liability of SBC at the level of gene.Results Theoretical studies found that the formation of blood stasis constitution was influenced by various of congenital and inherent factors,which included genetic factors, oldness,sex,tramatic injuries,seven emotions and internal injuries,improper diet, excessive labour and ease,and the prolonged disease.Genetic researches revealed that there were 592 differential gene locus expressed in blood stasis constitution group as compared with the balanced constitution group when P<0.05,which included 167 up-regulated gene locus and 425 down-regulated gene locus; When P<0.001,the differential gene locus expressed in blood stasis constitution group dcreased by 96 as compared with the balanced constitution group.Of these,24 were up-regulated gene locus and 72 were down-regulated gene locus.Patients with blood stasis constitution were more easily to have the platelet aggregation status,and had a high likelihood of suffering ischamic and tumor disease.A lot of biosynthesis-related genes which takes part in the process of protein,nucleic acid,and steroid hormone sythesis were down-regulated in population with blood stasis constitution,which indicates the decreasing status of genetic information transmission functions in blood stasis population.While the facts of many other genes which included growth and development-related and enzyme activity-related genes such as dihydropyrimidine dehydrogenase,human methyltransferase,protein-arginine n-methyltransferase,UDP glucose pyrophosphorylase,cholinephosphotransferase,phosphate galactose uridylic acid transferase,calpain,lactamase,down-regulated indicated again the possibility of decreasing in growth and development in population with blood stasis constitution.Conclusions The theoretical system of SBC could contribute to clinical medication and health preservation.There were significant differences of peripheral blood gene expression profile between the SBC and the normal constitution.The blood platelet of SBC is easier to aggregate and has the tendency to suffer from ischemic disease and cancer.The biosynthesis and metabolism function,the ability of genetic message transmission and the growth and development have downtrend.