Fufangjiakang Piece Of Graves' Disease In Rat T Cell Subsets And Cytokine Imbalance Experimental Study

Objective: GD is an autoimmune thyroid disorder involving on antibody(TsAb) directed against the TSH receptor (TSHR) producing thyroid stimulation. Tablet-FuFangJiaKang(Tablet-FFJK) is an empiric presciption of professor Ruquan Chen. It is eutherapeutic in clinic. In this experiment our objectivorder is to observe the unbalanced state of T cell subgroups in GD rat and the effect of adjustment after treated by Tablet-FuFangJiaKang.Methods:1.The animal models of GD were reconstructed: The wistar rats were mainlined 0.1ml, 0. 2ml, 0. 3ml, 0. 4ml, 0. 5ml Yersinia enterocolitica bacteriurm water(5× 108/ml) in trail in first,fifth,tenth, fifiteenth,and twentieth day.2. The change of the level of FT3, FT4, TSH in GD rats' blood serum and thyroid gland tissue pathology after treated with Tablet-FFJK of different dosage. Meanwhile, to compare the different effect of Tablet-FFJK, traditional Chinese medicine, and western medicine.3. We examine the proportion of CD4+,CD8+ T cell to show the state of Ts/Th by Flow Cytometry (FCM), and compare the effect of different group.4. We examine the proportion of CD25+ T cell to show the state of Tr by Flow Cytometry (FCM), and compare the effect of different group.5.We observe the state of Thl/Th2 by examine the cytokineses they excrete and compare the effect of different group. Result:1. The level of FT3, FT4 in GD rats' blood serum come forth higher and the TSH come forth lower after immuned with Yersinia enterocolitica bacteriurm water, and Tablet-FFJK can adjust it into normal.2. The Th/Ts state of GD rats come forth unbalance after immuned with Yersinia enterocolitica bacteriurm water, and Tablet-FFJK can adjust it into balance.3. The Tr state of GD rats come forth unbalance after immuned with Yersinia enterocolitica bacteriurm water, and Tablet-FFJK can adjust it into balance.4. The Thl/Th2 state of GD rats come forth unbalance after immuned with Yersinia enterocolitica bacteriurm water, and Tablet-FFJK can adjust it into balance.Conclution: In this experiment we observed that the unbalanced state of T cell subgroups in GD rat, and the Tablet-FuFangJiaKang could adjust its balance. Weinfer one of the mechanism is the Tablet-FFJK could reduce the level of thyroid hormone of GD rats' blood serum, and the other one is the traditional Chinese medicine could directly affect on the immune system by adjust the cytokineses.