| Objective :At the present situation,a great variety of cardiovascular diseases have more influence on the public group's physical and mental health,which affects deeply and profoundly,as well as reduced the quality of people's lives seriously.Coronary atherosclerotic heart disease(CHD)as a kind of relatively common,chronic diseases,as well as with the higher incidence among the other chronic heart diseases[1].Because the lipid metabolic abnormalities of the body leading to the coronary atherosclerosis,plaque formation,as well as the bureaucratic narrow arteries and blocking,that might be the intrinsical things of the pathological basis of CHD[3-4].There is existing a large number of studies have shown that human scavenger receptor sCD36 and low density lipoprotein cholesterol(LDL)is closely related to the metabolism,and it possibly can participate in the other lipids,let's provide an example for triglycerides,free fatty acids and the other in the metabolic process.By detecting the expression level of coronary heart disease patients' serum sCD36,understand its contact with lesions of coronary artery disease may be beneficial for those patients' clinical judgment and prognosis assessment,which is of such a great significance[5-6].Many domestic and foreign scholars' study shows that the serum hypersensitive c-reactive protein(hereinafter referred to as the Hs_CRP)as an inflammatory reaction medium with indirect degree of inflammation reaction and outcome,evaluations and other fatal and necessary role.Therefore,by the means of detecting serum Hs_CRP expression level,perhaps we can make an previous determination of the disease among those patients with CHD,and get an early understanding about our patients,founding the problems,and then make a perfect plan for our patients with timely examination and treatment,so as not to miss the best time to treatment,which is very important to delay the development of coronary heart disease for our patients mostly.The target of our study is to determinate the density of serum sCD36 among these patients with coronary artery illness,serum hypersensitive c-reactive protein(hs-CRP)expression level,to explore its relationship with coronary heart disease occurrence,development.Evaluate the clinical effectiveness and the value of application of these two indicators at the same time.Methods: 1.Cases receiving and collection: this study randomly selected those hospitalized patients from the ward of cardiovascular internal medicine of the number one ospital of Wuhan city,with 60 cases affirmed by CAG examination turns out coronary atherosclerosis in patients with coronary heart illness(CHD group),which are made up of the experimental group,there are 19 cases to a group of patients with unsteady angina pectoris(UAP sub-group);Group of 24 cases of steady angina group(SAP sub-group).17 cases of AMI sub-group,and then selected people who apply for physical examination in our hospital,30 cases of healthy candidates who's physical examination comes out to be normal and great will be classified as a under control group for our study.All research object in the group have to achieve routine inquiry about related history of illnesses,then physical inspection.We also record all subjects of sex,age,chronic illnesses give an example for hypertension,metabolic diseases or any other history of those illnesses,smoking history,and the rest of ordinary data.2.Specimen: all subjects of the study group have to be regular extraction on the hand day after admission,venous blood specimen we vested is ready for blood routine programs,renal function,Hs_CRP,the density of blood glucose under the condition with empty stomach,blood lipid and liver function index as well as blood coagulation routine check test etc.In addition,we will keep the rest venous blood of 3 ml,after coagulation and centrifugal get clear fluid,it is going to be kept in the refrigerator of-80 degrees Celsius,which is used to test the expression of serum sCD36 marked level.Among them,the consistency of Hs_CRP,which make use of latex enhanced immune turbidimetric method to determine and analysis instrument adopts fully Automatic biochemical Analyzer(Hitachi Automatic Analyzer,model 7060);sCD36 serum is obtained by the means of ELISA reagent method.The experimental detection products,adopt relevant reagents and related facilities,citing instrument alignment equipment,according to our instruction of the manual reagent,we are gonna to accomplish those specimen to be tested in a strictly way.By the time we clear the degree of those two makers,compare the expression of two index in the classification group,and then evaluate its correlation with CHD.3.Statistical data processing was executed by that software of SPSS 18.0 is provided to establish data base,input data parallel for data parsing and statistical management.All measurement data such as age,mean ± standard difference is in the implementation of ordinary data,and mean comparison between that double data samples were using t test measurement, nd the P values < 0.01,which might warning that difference has obvious significance of statistical subject,while the P values < 0.05,maybe prompt that differences equip significance of statistical subject.On the other hand,we'll study the factors which related with CHD to the single factor and multiple factors logistic regression analysis,when P < 0.05,it herald the difference is equipped with giant meaning on the subject of statistics.Whereas,those statistically significant factors will back to a multi-variable logistic regression processing of the model again,with P < 0.05 heralded the differences equip statistical essence.Result 1.The observation group of coronary heart disease(CHD group)in patients with serum sCD36 concentration was 163.44±91.72pg/m L,nevertheless,potency of serum sCD36 in the control group was 110.87±74.51pg/m L,the contrast between the display observation group index than the control group index expression increased(P<0.05),which indicates that the difference is equipped with enormous sense on the subject of statistics.2.The observation group coronary heart disease(CHD group)in patients with serum hypersensitive c-reactive protein concentration determination was 15.84±4.32mg/L,research objects in the control group shows that their serum hypersensitive c-reactive protein concentration determination was 2.98±0.57mg/L,which tells that the observation group than the control group indicators increased significantly(P <0.01),which shows that it has significant difference and with obvious statistical significance.3.The detailed group classification of observation group as the SAP sub-group,the UAP sub-group and the AMI sub-group.We find that the concentration of serum hypersensitive creactive protein and sCD36 of each group are higher than the control group in the varying degree,the hypersensitive c-reactive protein determination results in a significant statistical significance(P<0.01),serum sCD36 concentration determination by the statistical analysis was statistically significant(P < 0.05).4.The study of factors interconnection with CHD: through the single factor logistic regression analysis among data played among LDL,Hs_CRP,sCD36,smoking,hypertension as a risk factor for CHD(with P < 0.05);According to the results of single factor analysis,and then return those main factors above back to the model to finish the multiariable logistic egression analysis,consequences predict that the LDL,Hs_CRP,sCD36,smoking,hypertension are all independent essences of CHD.Conclusion 1.The level of sCD36 and Hs_CRP is obviously related to the occurrence of patients who are acquired CHD,which suggests these two makers can be obtained to be the risk factors of coronary atherosclerotic heart disease.The detection of serum sCD36 and serum hypersensitivity C-reactive protein levels may be helpful to predict the onset,evolution of coronary cardiac illnesses.Both are of practical significance in clinical diagnosis and treatment,risk estimation and prognosis evaluation of coronary cardiac diseases.2.LDL,hs_CRP,sCD36,history of smoking,hypertension are independent risk factors for coronary atherosclerotic heart disease. |
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