| BackgroundWith the development of economy and the improvement of people's living standards,the incidence and mortality of CHD have been increasing,and it is a major disease that seriously threatens people's life and health.The specific pathogenesis of coronary heart disease is not fully understood,but various theories exist,such as endothelial dysfunction,inflammatory reaction and lipid deposition hypothesis.Coronary heart disease is a complex systemic disease caused by a variety of factors,including hypertension,diabetes,dyslipidemia,autoimmune diseases.Recently,it has been found that intestinal flora may contribute to the occurrence of atherosclerosis by participating in the mediation of host oxidative stress,inflammatory response,and cholesterol metabolism disorder,and the metabolite of intestinal flora trimethylamine can promote the accumulation of cholesterol and the formation of foam cells,which can lead to the formation of vascular plaque.ObjectiveTo explore the correlation between the expression of homocysteine(Hcy),fibrinogen(FIB),high-sensitivity C-reactive protein(hs-CRP),and D-dimer(D-D)in patients with coronary heart disease.MethodFrom March 2016 to March 2019,124 patients admitted to the department of cardiology and intensive cardiac care unit(CCU)of the first affiliated hospital of xinxiang medical college who were diagnosed with coronary heart disease by coronary angiography were selected as the coronary heart disease group,and 47 healthy patients(who underwent coronary angiography or coronary CT)were selected as the control group.Subjects in the CHD group were admitted to the hospital in the early morning of the next day,and subjectsin the control group were examined on the day of physical examination.3ml elbow venous blood was extracted on an empty stomach.Serum FIB level was determined by thrombin enzyme method,serum Hcy level was determined by circulating enzyme method,and serum hs-crp and D-D levels were detected by immunoturbidimetry method.Fecal samples of subjects in the CHD group and control group were collected.Fresh fecal samples of subjects were collected in sterile frozen test tubes about 5.0g,sealed and sent to the key laboratory of heart disease within 1h.Then,2.00 g fecal matter was weighed and stored in the refrigerator at-80?.Concentration of extracting RNA and RNA detection,RNA transcribed into cDNA,fluorescence quantitative PCR,and finally isolated lactobacillus(Lac),bifidobacterium(Bif),escherichia coli(Eco),and calculate the per gram of feces in the bacteria on the number of living bacterium,the detection compared two groups of serum Hcy,FIB,hsCRP,D-D level and intestinal flora [lactobacillus(Lac),bifidobacterium(Bif),escherichia coli(Eco)levels,ROC curve analysis by using the serum index value to the diagnosis of coronary heart disease,Pearson was used to analyze the correlation between serum Hcy,FIB,hsCRP,D-D and Lac,Bif and Eco.Results1.Comparison of basic information between the two groupsThere was no significant difference in gender,age,BMI,occupation,smoking and alcohol consumption between the two groups(P >.05).The data between the two groups were comparable2.Comparison of serum Hcy,FIB,hs-CRP and d-d levelsThe serum levels of Hcy,FIB,hs-CRP and D-D in the CHD group were higher than those in the control group(P < 0.05),with statistically significant differences.3.The diagnostic value of serum levels for coronary heart diseaseThe AUC of serum Hcy in the diagnosis of coronary heart disease was greater than FIB,hsCRP,and D-D,up to 0.777,and the diagnostic sensitivity at the best cutoff value was 81.45%,specificity was 65.96%.4.Comparison of intestinal flora levels between the two groupsLac,Bif,and Eco levels in the coronary heart disease group were lower than those in the control group(P<0.05).5.Correlation between serum indexes and intestinal floraHcy was negatively correlated with Lac(r=-0.442,P<0.05),and negatively correlated with Bif(r=-0.490,P<0.05),negatively correlated with Eco(r=-0.506,P<0.05);hs-CRP was negatively correlated with Lac(r=-0.497,P<0.05),and negatively correlated with Bif(r=-0.548,P<0.05),negatively correlated with Eco(r=-0.522,P<0.05);FIB was negatively correlated with Lac(r=-0.496,P<0.05),and negatively correlated with Bif(r=-0.405,P<0.05),negatively correlated with Eco(r=-0.408,P<0.05);D-D was negatively correlated with Lac(r=-0.584,P<0.05),and negatively correlated with Bif(r=-0.553,P<0.05),which was negatively correlated with Eco(r=-0.508,P<0.05).ConclusionThe serum Hcy,FIB,hs-CRP,and D-D expressions of patients with coronary heart disease are abnormally elevated,and the intestinal flora is disordered.There is a significant negative correlation between the two. |
PDF Full Text Request