| Objective:To investigate whether Periplaneta americana L.Extract(PAE)could promote wound healing after diabetic anal fistula operation by down-regulating LINC01133 and its target gene SLAMF9,and to analyze the healing mechanism Periplaneta americana L.Extract,Methods;The long non-coding RNAs(lncRNAs)expressions were analyzed by high-throughput sequencing in wound tissues from diabetic anal fistula patients with or without PAE treatment.Peripheral blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor(hM-CSF),then M1 macrophages was induced by lipopolysaccharide,Macrophage markers were detected by Flow cytometry.Cell viability,migration and angiogenic capacity were assessed in human umbilical vein endothelial cells(HUVEC)co-cultured with macrophages using CCK-8,cell scratch and tube formation assays.RIP and RNA pull-down were operated for confirming interactions between genes.Results:LINC01133 and SLAMF9 were downregulated in wound tissues of PAE-treated patients,Periplaneta americana L.Extract suppressed M1 macrophage polarization and promoted HUVEC proliferation,migration and angiogenesis,which were reversed by overexpression of LINC01133 or SLAMF9,LINC01133 upregulated SLAMF9 through interacting with ELAVL1.SLAMF9 overexpression overturned the effects of LINC01133 silencing on macrophage polarization and HUVEC.Conclusion:Periplaneta americana L.Extract promoted wound healing after diabetic anal fistula operation by downregulating LINC01133/SLAMF9. |
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