| Objective:Using modern data mining technology to analyze the characteristics and medication patterns of traditional Chinese medicine prescriptions for treating knee osteoarthritis(KOA)based on the pathogenesis of blood stasis and dampness,including drug properties,meridians,core drug groups,etc,in order to provide reference for future clinical prescriptions and medication.Methods:This study sets the theme words as "knee osteoarthritis","osteoarthritis","knee bi",etc,and combines the free words "stasis dampness","phlegm stasis","phlegm dampness","blood stasis",etc.to search for the target literature in a free combination on China National Knowledge Infrastructure,VIP full-text database,Wanfang database,China Biomedical Literature Database,and Youxiu Academic Database,respectively,Then count the literature retrieved from each database.Merge all the obtained literature and remove duplicates to obtain the remaining research literature.Then,based on the inclusion and exclusion criteria,relevant literature is screened again to obtain literature that meets the requirements for mining traditional Chinese medicine prescriptions.By reading the title and abstract of each literature,and carefully reading the entire text,the traditional Chinese medicine prescriptions in the literature are organized,and a prescription Chinese medicine database for treating KOA is established that is included in the mining analysis.Then query the four qi,five flavors,and meridians of each traditional Chinese medicine,and use Excel 2016 software to establish a statistical table.According to the established traditional Chinese medicine database,frequency statistics were conducted using Excel 2016 software to screen high-frequency drugs.Then,use IBM SPSS 25.0 and IBM SPSS Modeler 18.0 statistical software for system clustering analysis and association rule analysis,and create relevant visual network diagrams.Finally,compare and analyze the data results obtained from the analysis with the Huayu Qushi Formula studied by the supervisor in the previous stage.Results:This study ultimately included 59 traditional Chinese medicine prescriptions and conducted data analysis,resulting in a total of 157 traditional Chinese medicines.Using Excel 2016 software,the frequency of use of all traditional Chinese medicines was statistically analyzed,and the cumulative frequency of each Chinese medicine occurrence was 700 times.There are 28 Chinese herbal medicines with a frequency of ≥ 8 in each prescription,and the top ten Chinese herbal medicines with a frequency of use are Angelica sinensis,Achyranthes bidentata,Salvia miltiorrhiza,Poria cocos,safflower,Chuanxiong,licorice,mustard,Atractylodes macrocephala,and Angelica dahurica.The nature,taste and meridian tropism of each Chinese medicine were statistically analyzed,and the relevant radar chart was drawn.The research results of the Four Qi show that the total frequency of the Four Qi in all traditional Chinese medicines is 700 times,with the highest being warm(297times,42.4%),followed by mild(177 times,25.2%),cold(72 times,10.2%),mild(61 times,8.7%),mild(60 times,8.5%),cool(21 times,3%),hot(7times,1%),and high(5 times,0.7%).The results of the Five Flavors study showed that the highest frequency of occurrence among all traditional Chinese medicines was pungent(342 times),followed by bitter(311 times),sweet(280 times),salty(58 times),light(52 times),slightly bitter(49 times),sour(39 times),slightly sweet and astringent(5 times),slightly spicy(3times),and slightly astringent(1 time).The results of the study on meridian tropism showed that the main Chinese herbal medicines included in the study were the liver meridian of Foot Jueyin(417 times,24.1%),followed by the spleen meridian of Foot Taiyin(306 times,17.7%),the heart meridian of Hand Shaoyin(221 times,12.7%),the kidney meridian of Foot Shaoyin(213times,12.3%),the lung meridian of Hand Taiyin(210 times,12.1%),the stomach meridian of Foot Yangming(163 times,9.4%),the bladder meridian of Foot Taiyang(91 times,5.2%),and the gallbladder meridian of Foot Shaoyang(35 times,2.0%)Hand Yangming Large Intestine Meridian(32times,1.8%),Hand Jueyin Pericardium Meridian(24 times,1.3%),Hand Taiyang Small Intestine Meridian(8 times,0.46%),Hand Shaoyang Triple Jiao Meridian(7 times,0.4%).According to the clustering analysis of high-frequency drugs,it can be divided into 5 effective clustering combinations of traditional Chinese medicine,namely Cluster I(Atractylodes macrocephala,Fangji,Poria cocos,Atractylodes macrocephala,Safflower,Chuanxiong,Citrus reticulata,Pinellia ternata,Mustard seed,Achyranthes bidentata,and Caulis spatholobi),Cluster II(Salvia miltiorrhiza,Coix seed,and earthworm),Cluster III(Peach kernel,White peony root,and licorice root),Cluster IV(Weilingxian,Zelan,and Achyranthes bidentata),Cluster V(Angelica sinensis,Astragalus membranaceus,Rhizoma Notopterygii,Rhizoma Angelicae,and Gentiana macrophylla).Through the analysis of drug association rules,it was found that the top 5 drug combinations with support were safflower → Chuanxiong(33.89%),Angelica → Chuanxiong(33.89%),Poria cocos → Atractylodes macrocephala(28.81%),Angelica → Chuanxiong,safflower(28.81%),Chuanxiong → safflower,and Angelica(28.81%).Among them,the drugs in cluster V have strong overlap with Juanbi Tang.In the drug group,Gentiana macrophylla,Radix Angelicae Dahuricae,and Rhizoma Notopterygii can dispel rheumatism,unblock collaterals,and relieve arthralgia pain.Angelica sinensis and Astragalus membranaceus belong to the category of tonifying deficiency drugs,which can not only tonify qi and blood but also promote blood circulation and circulation,and have the function of relieving arthralgia pain.Comparing high-frequency traditional Chinese medicine with the prescription for removing blood stasis and dampness,it was found that up to eight traditional Chinese medicines overlap.At the same time,it was found that the same medicine mainly has a warm and bitter taste,and mainly enters the liver meridian of Foot Jueyin.Conclusion:1.In the early stages of knee osteoarthritis,it is mainly characterized by pathogenic factors such as wind,cold,dampness,heat,and blood stasis;In the late stage of this disease,it is mostly a deficiency syndrome,or a combination of deficiency and excess.It is common to have insufficient qi and blood,liver and kidney,or mixed with solid pathogens such as phlegm,heat,and blood stasis.2.The traditional Chinese medicine for treating KOA based on the pathogenesis of dampness and stasis is mainly composed of Angelica sinensis,Huai Niu Xi,Danshen,Poria cocos,safflower,and Chuanxiong,with the majority of warm and hot herbs.The five flavors are mostly bitter and spicy,mainly entering the liver meridian of Foot Jue Yin.3.By comparing the high-frequency traditional Chinese medicine obtained through data mining with the mentor’s empirical formula-Huayu Qushi Fang,it was found that the two have a high degree of overlap,with the same traditional Chinese medicine reaching up to eight flavors.This can provide a theoretical basis for the rationality of Huayu Qushi Fang’s formulation,and also provide guidance and reference for the clinical differentiation and medication of young medical students. |
PDF Full Text Request