Effects Of Adding Curcumin And Resveratrol To Periparturient Diets On Production Performance,Blood Indexes And Gene Expression Of Inflammatory Factors In Does

In the periparturient period,does tend to show negative energy balance,because the fetus grows and the rumen is compressed,and the food intake of the does is reduced.At the same time,the nutritional requirements of offspring have increased sharply.Does need to mobilize stored body fat,and a large amount of ROS will be produced during the energy supply process of the liver,which leads to the occurrence of oxidative stress and induces adverse reactions such as metabolic diseases and inflammation.Curcumin and resveratrol have the functions of improving immunity,lipid metabolism,antibacterial and antiviral.They have been used in animal husbandry production to improve the quality of animal products and production performance,but few reports in the periparturient period.Therefore,in this study,goats in the periparturient period were fed with diet including curcumin or resveratrol,and blood biochemistry and hormone determination,real-time quantitative PCR and other technologies were applied to study their effects on goat performance,blood indicators and inflammatory gene expression,which provide scientific basis for their application in periparturient production.1.Effect of adding curcumin to the periparturient diet on the production performance,blood index and inflammatory factor gene expression in does.Eighteen Subai does pregnant for 122 days(28d before excepted delivery)were divided into Con group(without addition)and Cur group(with curcumin 800mg/kg DMI).Blood was collected at-21 d,0d,and 21 d.Production performance,blood indicators,and inflammatory factor gene expression were determined.The results showed that Times had a significant trend on the feed intake of the two groups(P=0.097).The feed intake range of the two groups decreased first and then increased,and the feed intake was the lowest on the day of delivery;the addition of curcumin significantly reduced the abortion rate(P<0.05).There was no significant effect on lamb birth weight,body size and average daily gain at21 days of postpartum(P>0.05).In terms of biochemical indicators: Times had a significant impact or trend on low-density lipoprotein,high-density lipoprotein,total cholesterol and triglycerides,while treatment factors and interactions were not significant;Curcumin treatment significantly reduce NEFA content(P=0.041);the interaction factors of Times and treatment x Time also had an influence on NEFA(P<0.1).Antioxidant indicators: the levels of SOD and T-AOC in the Cur group were increased;the content of MDA was mainly affected by Times;the change of GSH-Px was not significant.In terms of immune indicators,curcumin treatment significantly affected the Ig A content(P<0.05),and there was no significant change in the Ig G content(P>0.05).Times tended to influence the Ig M content(P=0.063).Hormonal indicators: treatment factors had a significant impact on the content of ADPN(P<0.05);also,the content of COR and progesterone was affected by Times.Adding curcumin significantly increased the expression of Nrf2 on the 21 day of postpartum(P<0.05),and decreased the expression of TNFα on the 0 day of postpartum(P<0.05),but IL-1,IL-6 and IFNγ had no significant difference.All these results indicate that adding curcumin to the periparturient diet can improve the lipid metabolism of the does during the periparturient period,enhance the anti-oxidant performance of the does,and reduce the inflammatory response.2.Effects of resveratrol supplementation in periparturient diet on production performance,blood indexes and gene expression of inflammatory factors in doesEighteen Subai does pregnant for129 days(21d before excepted delivery)were divided into Con group(no supplementation)and Res group(addition of resveratrol600mg/kg DMI).Blood was collected at-15 d,0d,and 15 d.Production performance,blood indicators,and inflammatory factor gene expression were determined.The results showed that Times had a significant effect on the feed intake of the two groups(P=0.023),which was the lowest on the day of delivery;the addition of resveratrol had no significant effect on the birth weight,daily gain and birth size of the offspring of does(P >0.05).In terms of biochemical indicators: the interaction of treatment X time tends to influenced HDL,and HDL in Res group was significantly higher than the Con group at 0 days postpartum(P=0.05),and it tended to be higher than Con at 15 days postpartum(P=0.07);Times,treatment factors and interactions all had an impact on the content of NEFA.The addition of resveratrol significantly reduced the content of NEFA at 0 days postpartum;treatment factors had a trend of influencing total cholesterol(P= 0.093);Times had a very significant effect on triglycerides(P=0.002);while there was no significant change in low-density lipoprotein and glucose during the test.Antioxidant indicators: treatment factors significantly increased the levels of SOD and T-AOC in the Res group;the contents of GSH-Px and MDA did not change significantly.In terms of immune indicators,resveratrol treatment significantly affected the content of Ig A and Ig M(P<0.05),but the Ig G content did not change significantly during the experiment(P>0.05).In terms of hormones indicators: the content of ADPN and progesterone was affected by Times,and the content of COR did not change significantly during the test period(P>0.05).Adding resveratrol had a tendency to reduce the expression of TNFα and IFNγ at 15 days of postpartum(P<0.1),but IL-1 and IL-6 had no significant difference.All these results indicate that dietary supplementation of resveratrol can improve the fat metabolism of does during the periparturient period,enhance antioxidant performance,and reduce inflammation.