| Common gastrointestinal infections in dogs and cats include canine/feline parvovirus,canine/feline coronavirus,canine/feline rotavirus and canine distemper virus,which usually cause gastrointestinal symptoms in dogs and cats,such as decreased appetite,abstinence,vomiting and diarrhea,which can range from mild to severe,and some infected cases are accompanied by the risk of death.At present,there is no report on the epidemiology of common gastrointestinal infections in dogs and cats in Wuhan,so it is urgent to carry out investigations on the above-mentioned virus infections in this area.From August 2020 to July 2021,a total of 414 dog and cat samples with clinical manifestations of diarrhea and healthy were collected from several animal hospitals in Wuhan,including 192 dog samples and 222 cat samples.At the same time,the clinical details of the recorded samples were collected.The epidemiology situation of common gastrointestinal infection viruses in dogs and cats in Wuhan was systematically analyzed,and the positive cases of canine parvovirus were sequenced and typed,as well as the sequencing and genetic evolution analysis of feline parvovirus VP2 gene.These data provide a theoretical reference for the prevention and control of common gastrointestinal infections in dogs and cats in Wuhan.The contents results of this study are as follows:1.Epidemiological investigation of common infection viruses in canine digestive tract in Wuhan areaThe collected 192 dog samples were subjected to PCR detection of related viruses,then combined with clinical case information to conduct virus epidemiological investigations in Wuhan.The infection rate of CPV in dogs in Wuhan was 46.35%(89/192).The infection rate of female dogs was higher than male dogs,and the infection rate of puppies within 3 months was the highest,but there was no relationship between the infection and sex(p=0.813)and age(p=0.802),and the incompletely immunized dogs were more susceptible to infection than the completely immunized dogs(p=0.004).The infection rate is higher in spring and autumn,but there is no correlation with the season(p=0.157).The infection rate of CCo V was 25.00%(48/192),the infection rate of female dogs was higher than male dogs,but there was no correlation between CCo V infection and sex(p=0.933).Puppies within 3 months of age were more susceptible to CCo V infection than dogs of other age(p<0.001),and incompletely immunized dogs were more susceptible than completely immunized dogs(p=0.002).The infection rate was higher in autumn and winter,but there was no significant correlation between CCo V infection and season(p=0.432).The infection rate of CDV was 8.33%(16/192).The infection rate of male dogs was higher than female dogs,but the infection is not related to sex(p=0.177).Puppies within 3 months of age were more susceptible to CDV infection than dogs of other age(p<0.001),and incompletely immunized dogs were more susceptible than completely immunized dogs(p=0.001).The infection rates were higher in summer and winter,but there was no correlation between the infection and season(p=0.469).The infection rate of CRV was 40.63%(78/192).The infection rate of female dogs was higher than that of male dogs,and the infection rate of puppies within 3 months was the highest,but there was no correlation between the infection with gender(p=0.235)and age(p=0.241).CRV infection was more likely occurs in spring and summer(p<0.001).The common digestive tract infection viruses in dogs include single infection and mixed infection of multiple viruses.The highest positive rate of mixed infection of CPV and CRV was 14.58%(28/192),and there was no mixed infection of CPV and CDV.2.Epidemiological investigation of common infection viruses in feline digestive tract in Wuhan areaThe collected 222 cat samples were subjected to PCR detection of related viruses,and then combined with clinical case information to conduct virus epidemiological investigations in Wuhan.The infection rate of FPV among cats in Wuhan was 25.23%(56/222),the infection rate of male cats was higher than female cats,but the infection rate was not associated with sex(p=0.354).Kittens within 3 months of age were more susceptible to FPV infection than cats of other age(p=0.038),and incompletely immunized cats were more susceptible(p=0.034).The infection rate was higher in spring and autumn,and the infection rate was correlated with season(p=0.003).The infection rate of FCo V was 38.29%(85/222).The infection rate of male cats was higher than female cats,but there was no correlation with gender(p=0.679).The infection rate of kittens within 3 months of age was higher,but FCo V infection was not related to age(p=0.157).The infection rate was higher in autumn and winter,but the season was not related to infection(p=0.165).The infection rate of FRV was 52.70%(117/222).The infection rate of female cats was higher than male cats,feline at 4-7 months had the highest infection rate of FRV infection,and the infection rate of FRV was higher in spring and winter.But there was no correlation between FRV infection and sex(p=0.347),age(p=0.589)and season(p=0.182).The common digestive tract infection viruses in cats include single infection and mixed infection of multiple viruses.The highest positive rate of FCo V and FRV mixed infection was 13.96%(31/222).3.Molecular epidemiological investigation of CPV and FPVThe CPV-positive dogs detected by PCR method were further classified,and the CPV-2 typing results were obtained after sequencing.Among the CPV-positive dogs in Wuhan,20 were CPV-2a and 61 were CPV-2c,CPV-2b did not appear.FPV-positive cats detected by PCR method were further detected and sequenced based on the VP2 gene,and the phylogenetic tree was constructed.According to the results,it was found that the prevalent FPV strains in the country,including Wuhan,belonged to G1 and G3 types,which were closely related to the vaccine strains and their locations.The G2 type is more distantly related.To sum up,this study preliminarily revealed the prevalence of canine and feline parvovirus,canine and feline coronavirus,canine and feline rotavirus and canine distemper virus in Wuhan through an epidemiological survey of common gastrointestinal infections in dogs and cats in Wuhan.It provides data support for clinical diagnosis and treatment,and provides a theoretical basis for rotavirus as a zoonotic virus to be paid attention to and in-depth research.At the same time,it also provides suggestions for better prevention and control of common gastrointestinal infections in dogs and cats in the region. |
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