Research On Water Environment Monitoring Of Weihai Port Based On Metagenomic Technology

Ports are important driving force of economic growth as well as the major source of energy consumption and pollution.With new situations of global energy crisis and environmental degradation,port industry puts forward the concept of sustainable,green and ecological development to obtain a good balance between environmental and economic benefits in the face of globalized economic forms and the trend of regional resource integration in conglomerates.China has released development strategy of ports focused on structural reform of supply-side to build a resourcesaving and environment-friendly green development system,providing stronger support for construction of green ports.As a major maritime province,Shandong ports have completed integration and restructuring,and it has become imperative to improve the economic-ecological coupling evaluation analysis of ports,construct a green port evaluation index system and conduct scientific and reasonable evaluation of ports.However,traditional methods of determining green ports using grade of emission indicator values has a high degree of blindness and uncertainty.Therefore,adding quantitative analysis of water environment monitoring to the green port grade evaluation system is conducive to improvement of the evaluation system.This paper selects Weihai port as the research model port to study the port water environment monitoring and green development based on metagenomic technology.High-throughput metagenome sequencing technology was applied to sequence the metagenome of the water environment in Weihai port and to analyze the composition of the microbial community and antibiotic resistance genes(ARGs)in the water.Furthermore,this paper selects microbial communities and antibiotic resistance genes in the global representative coastal environment and uses a comparative metagenomic approach to investigate the correlation and difference with the diversity of Weihai port.Finally,this paper evaluates the Weihai water environment and its impact on human health and port transportation with quantitative,comparative,literature,and empirical research methods.The research results are as follows: Firstly,metagenomic sequencing technology provides effective information for microbial community and antibiotic resistance gene in water environment of Weihai port.Secondly,the dominant species in the port water environment in this study are Rhodobacteraceae,Gammaproteobacteria and Bacteroidetes.Meanwhile,multidrug,tetracycline and MLS antibiotic resistance genes are dominant ARGs.Besides,the water conditions in beach waters are better than those in port waters.Thirdly,through the analysis of comparative metagenomic data,it shows that the dominant species analysis and antibiotic resistance genes can be used as powerful indicators to monitor the water environment.The species abundance reflects the abundance of flora in the water environment,however,it cannot be used as an indicator to judge the pollution degree of water alone.Finally,port transportation and port water environment are closely related and interact with each other in a dynamic coupling relationship.The tetracycline resistance gene in the water environment of Weihai port is slightly higher due to the surrounding aquaculture,pharmaceutical wastewater,domestic sewage,oily sewage and ship ballast water.Therefore,prevention,monitoring and governance need to be strengthened to ensure the benign development of port transportation industry.This paper will provide technical and theoretical support for the management of waters of Weihai port as well as be popularized and applied to urban monitoring of microbiological risk of port waters,offer scientific guidance for restoration and reconstruction of the port ecosystem and promote improvements of the green port evaluation system.