Prediction Of Antioxidant Enzyme Gene Polymorphisms On Aerobic Capacity And Training Effect Of College Students

Research objectives:By analyzing the effect of antioxidant enzyme gene polymorphisms on aerobic capacity phenotypic indexes before and after high-intensity interval training（HIIT）intervention,molecular genetic markers for predicting aerobic capacity and training effect were explored,which provided a basis for formulating precise exercise prescriptions.Research objects and methods:In this study,245 Han college students were selected for 12 weeks of HIIT intervention（3 times/week）.Before and after the intervention,800/1000m running performance,maximum oxygen uptake（VO₂max）,ventilation threshold（VT）,and energy saving（EE）were tested,and venous blood was extracted to extract DNA and genotyping the CAT,Mn-SOD,GPx1,and GSTP1 genes.Plink 1.90 and Haploview4.2 were used to screen positive loci consistent with H-W homeostasis and associated with aerobic capacity.Differences in aerobic capacity between carriers of different dominant allele numbers were analyzed using a multifactorial ANOVA.The degree of explanation of the genetic predisposition score（GPS）for the differences in the relevant indexes and the construction of the prediction model were analyzed by stepwise regression,and the optimal GPS threshold for predicting aerobic capacity and training effect was analyzed by ROC working curve.Research results:1.CAT genes rs10836235 and rs524154 were significantly correlated with the change rate of 800/1000m running performance,and the interpretation degree of GPS（rs10836235+rs524154）for the change rate of 800/1000m running performance was 1.8%.The 800/1000m performance reduction of GPS（rs10836235+rs524154）3≥.1 carriers was significantly higher than that of GPS（rs10836235+rs524154）<3.1.2.CAT gene rs10836235,GPx1 gene rs1050450,GSTP1 gene rs762803,rs1695 were significantly correlated with HR max basic values,GPS（rs10836235+rs1050450+rs762803+rs1695）explained the difference in HR max basic value by4.7%.The basic value of HR max for carriers of GPS（rs10836235+rs1050450+rs762803+rs1695）≥4.191 is significantly higher than that of carriers of GPS（rs10836235+rs1050450+rs762803+rs1695）<4.191.3.Mn-SOD genes rs5746136 and rs732498 were significantly correlated with the change rate of VE under VO₂max test,GPS（rs5746136+rs732498）explained the difference in VE rate of change by 1.9%.The VO₂max-VE increase in carriers of GPS（rs5746136+rs732498）≥7.113 was significantly higher than that of carriers of GPS（rs5746136+rs732498）<7.113.4.Mn-SOD genes rs5746136,rs2758339 and rs732498 were significantly correlated with VO₂max（L/min）change rates,GPS（rs5746136+rs2758339+rs732498）explained the difference in VO₂max（L/min）rate of change by 2%.The VO2max（L/min）increase in carriers of GPS（rs5746136+rs2758339+rs732498）≥6.457 was significantly higher than that of carriers of GPS（rs5746136+rs2758339+rs732498）<6.457.5.GPx1 gene rs1050450 was significantly correlated with the change rate of EE-HR,The reduction in EE-HR was significantly higher in GG genotype carriers than in AG genotype carriers.6.GSTP1 genes rs762803 and rs4147581 were significantly correlated with the basic value of EE-VE,GPS（rs762803+rs4147581）explained the difference in the basic value of EE-VE by 1.3%.There was no significant difference between the carrier EE-VE base values for GPS（rs762803+rs4147581）≥6.802 and the carrier EE-VE base value for GPS（rs762803+rs4147581）<6.802.7.GSTP1 genes rs762803 and rs4147581 were significantly correlated with the basic value of EE-VO₂（L/min）,the difference in the base value of EE-VO₂（L/min）was explained by GPS（rs762803+rs4147581）by 0.1%,and there was no significant difference between the carrier of GPS（rs762803+rs4147581）≥1.565 and the carrier EE-VO₂（L/min）base value of GPS（rs762803+rs4147581）<1.565.8.Mn SOD genes rs5746136 and rs732498 were significantly correlated with the change rate of EE-VO₂（L/min）,GPS（rs5746136+rs732498）explained the difference in the rate of change of EE-VO₂（L/min）by 1.1%.The reduction in EE-VO₂（L/min）for carriers of GPS（rs5746136+rs732498）≥3.918 was significantly higher than that for carriers of GPS（rs5746136+rs732498）<3.918..9.GSTP1 genes rs762803 and rs4147581 were significantly correlated with the basic values of EE-VO₂（ml/min/kg）,GPS（rs762803+rs4147581）explained the difference in EE-VO₂（ml/min/kg）base values by 1.3%.There was no significant difference between the carrier EE-VO₂（ml/min/kg）values of GPS（rs762803+rs4147581）≥2.956 and the carrier EE-VO₂（ml/min/kg）base value of GPS（rs762803+rs4147581）<2.956.10.Mn SOD genes rs5746136 and rs732498 were significantly correlated with the change rate of EE-VO₂（ml/min/kg）,GPS（rs5746136+rs732498）explained the difference in the rate of change of EE-VO₂（ml/min/kg）by 1.1%.The reduction in EE-VO₂（ml/min/kg）of carriers of GPS（rs5746136+rs732498）≥4.692 was significantly higher than that of carriers of GPS（rs5746136+rs732498）<4.692.Research conclusions:1.CAT gene GPS（rs10836235+rs524154）can predict the training effect of 800/1000m running performance after 12 weeks HIIT intervention.2.CAT,GPx1,GSTP1 gene GPS（rs10836235+rs1050450+rs762803+rs1685）can predict the initial level of HR max before exercise intervention;The GPx1 gene rs1050450 can be used as a genetic marker for the effect of EE-HR training after 12 weeks HIIT training.3.Mn-SOD gene GPS（rs5746136+rs732498）can predict the training effect of VO₂max-VE,EE-VO₂（L/min）and EE-VO₂（ml/min/kg）after 12 weeks HIIT intervention;GPS（rs5746136+rs2758339+rs732498）can predict the training sensitivity of VO₂max（L/min）after12 weeks HIIT intervention.