Study On The Characteristics Of Intestinal Flora In Diarrhea Mice With Liver-qi Over Spleen Syndrome

Objective: To analyse the effect and investigate the characteristics of the intestinal flora of mice with Ganqichengpi diarrhea caused by Folium senna decoction gavage combined with restraint and tail pinch stress.Methods: Ten healthy male Kunming mice were chosen and randomly divided into control group and model group,with five mice in each group.Mice in the control group were raised regularly,while mice in the model group were treated for 7 days by feeding with 1 g/m L Folium senna decoction once a day,restraint in a constraint tube of 50 m L volume and tail pinch with a clip at the same time for 1 hour to carry out the model of Ganqichengpi diarrhea.Intestinal contents from the jejunum to ileum were collected,and DNA was extracted from each mouse.The characteristics of the intestinal microbial species were analysed by Pac Bio Sequel-based 16 S r RNA sequencing.Results: Alpha diversity indices in the model group were higher than those in the control group,and the Simpson index differed significantly(P < 0.05).Based on the composition and abundances of species,there were differences between the control group and model group at the species level,but these differences were not significant(P > 0.05).In the control group,Candidatus arthromitus sp.SFB-mouse and Lactobacillus johnsonii were the dominant species.In the model group,Staphylococcus lentus,Lactobacillus johnsonii,Candidatus arthromitus sp.SFB-mouse and Lactobacillus murinus were included.Furthermore,LEf Se analysis showed that the relative abundances of Escherichia sp.BBDP27,Helicobacter ganmani,Bacteroides vulgatus and Lactobacillus intestinalis in the model group were higher than those in the control group(P < 0.05 or P < 0.01).Conclusion: Ganqichengpi diarrhea caused by Folium senna decoction gavage combined with restraint and tail pinch stress increased the intestinal flora evenness and diversity.Strains associated with intestinal diseases,including Bacteroides vulgatus,Helicobacter ganmani,Staphylococcus lentus and Lactobacillus murinus,were significantly enriched,while strains beneficial to health,such as Candidatus arthromitus sp.SFB-mouse and Lactobacillus johnsonii,were significantly depleted.Bacteroides vulgatus 、 Lactobacillus intestinalis 、 Escherichia sp.BBDP27 and Helicobacter ganmani could be considered as characteristic flora of Ganqichengpi diarrhea.