Effects Of Long-term Aerobic Exercise On Energy Intake And Energy Consumption In Normal And Obese Mice

PurposeExercise is an effective means to lose weight,but the weight loss effect of pure exercise is lower than people’s expectations,and there are individual differences.Some studies believe that exercise can cause a negative energy balance and reduce weight.Other studies have proven that there is corresponding energy compensation to resist weight loss after exercise.Considering the controversy,this experiment conducted a20-week aerobic exercise intervention on normal and obese mice.The purpose of this study was to investigate the effects of long-term aerobic exercise on the energy intake and energy expenditure of normal-weight and obese mice.Analyze the reasons to provide theoretical basis for exercise weight loss.MethodThis study was conducted on 52 mice.They were randomly divided into normal diet group(24 animals)and high-fat diet group(28 animals).The first 16-week model of nutritional obesity was performed.After 16 weeks of feeding,4 mice that failed weight removal were weighed and randomly divided into normal quiet group(12 animals)and normal exercise group(12 animals),obese quiet group(12 animals)and obese exercise group(12 animals).The exercise group was subjected to aerobic exercise intervention for20 weeks(6 times a week,35 minutes each time).After the intervention,the body weight,body composition,energy intake,energy expenditure,basal metabolism and activity level of the mice were measured.ResultNormal mice: There was no significant difference in body weight and lean body mass in the exercise group compared to the quiet group;body fat% decreased significantly(P<0.05);there was no significant difference in energy expenditure,basal metabolism,respiratory quotient,and activity levels;leptin mRNA expression Very significant decrease(P <0.01);there is no significant difference in leptin receptor mRNA.Obese mice: There was no significant change in lean body mass in the exercise group compared with the quiet group,and the body weight was significantly reduced(P <0.01);body fat% was significantly reduced(P <0.05);energy intake was significantly reduced(P<0.05);energy consumption,There was no significant difference in basal metabolism,respiratory quotient and activity level;leptin gene expression increased significantly(P<0.01);leptin receptor mRNA increased significantly(P <0.05).Obese and normal mice: the obese quiet group had significantly higher lean body mass and body fat% than the normal quiet group(P <0.01);energy intake increased significantly(P <0.05);energy expenditure and basal metabolism were significantly increased High(P <0.01);respiratory quotient decreased significantly(P <0.01);leptin mRNA expression decreased significantly(P <0.05);leptin receptor mRNA did not change significantly.Compared with the normal exercise group,the body weight and body fat% of the obese exercise group were significantly increased(P <0.01);the lean body mass was significantly increased(P <0.05);there was no significant difference in energy intake,and energy consumption and basal metabolism were very significant Elevated(P <0.01);Respiratory quotient decreased significantly(P <0.01);Leptin mRNA increased significantly(P <0.01);Leptin receptor mRNA increased significantly(P<0.05).Conclusion(1)Long-term aerobic exercise has a significant effect on weight loss in normal weight mice without weight loss,while it has a very significant effect on weight loss and fat loss in obese mice.(2)The reason for the long-term aerobic exercise to reduce the energy intake of obese mice may be related to the increased expression of leptin and leptin receptor mRNA.