Effective Components From Momordica Grosvenori Contribute To The Relieve Of Immunosuppression

Momordica grosvenori(Luo Han Guo),a specialty in northern area of Guilin province was an excellent source for elements,including mogrosides,momordica polysaccharide,fatty acids,protein amino acids and flavonoid glycoside.Characterized by cough relieve and sputum decrease,this fruit was beneficial in promoting digestion,protecting liver and lowering transaminase,preventing and treating diabetes,regulating immunity as well as promoting blood circulation for removing blood stasis and removing free radical and promoting antioxidant activity.This study was conducted by four weeks of high-intensity training and intervention was performed using effective components from Luo Han Guo.Then indexes of immune function were measured between two training groups to investigate whether effective components from Luo Han Guo can relieve and eliminate the immunosuppression during high-intensity training.Our study aims to provide theoretical basis for regulation on decline in immunological functionMethod:Our study included a total of 24 senior ordinary students or senior students majored in physical education(PE),which was randomly grouped into control group(no training no treatment,n=8),training group(training without treatment,n=8)and Luo Han Guo intervention group(training without treatment,n=8).Luo Han Guo intervention group was administrated with 8 ml/kg/d of effective components from Luo Han Guo at the moment of 15 min before training and the end of training.Meanwhile,control group was given for the same amount of purified water at the same moment.Both of the two training groups were arranged for four weeks of high intensity training,mainly including anaerobic training.The training intensity was controlled around 85%and was mainly determined on heart rate and blood lactic acid levels.Training was conducted twice a day with 90 min for each time.The training time was 7:00～8:00 am and 4:00～5:30 pm.No training was arranged on each Sunday.Then after the training course,venous blood after overnight fasting was collected respectively on Monday 7:00 am on 1st week and 3nd week,as well as 7:00 am on Sunday on 4th week.Subsequently,the blood sample was anticoagulated with EDTA and centrifuged to separate the supernatant,followed by preservation on a refrigerator at-20 C.In addition,peripheral blood,immune globulin and T lymphocyte subsets were also detected.Results:(1)No significant difference was observed on WBC,LYM#and MONO#between training group and Luo Han Guo intervention group regarding to all time points(P>0.05).The WBC in training group was decreased in comparison to Luo Han Guo intervention group at the end of training,failing to achieve statistical significance(P>0.05),while the MONO#in training group was remarkably elevated after 4 weeks of training compared with that in the control group(P<0.05).Our results also found that NEU#in training group was notably decreased at the end of training in contrast to Luo Han Guo intervention group and control group(P<0.05).Comparisons on different time points showed WBC in training group at the middle of training was increased compared with that before training(P<0.05),while MONO#was presented with a slightly decreased but a sharp increased trend during training courses(P<0.01).The NEU#after training was significantly reduced compared with that before training(P<0.05).In Luo Han Guo intervention group,MONO#was remarkably increased with the increased of training time(P<0.01).(2)At the middle of the training,the IgG in the training group was significantly decreased compared with control group(P<0.05).At the middle and end of the training,significant difference on IgG was detected between training group and Luo Han Guo intervention group(P<0.05).We also found that IgG in the Luo Han Guo intervention group was elevated with the increase of training time(P<0.05).At the end of the training,the IgM in training group was significantly decreased compared with control group and Luo Han Guo intervention group(P<0.05).The IgA in training group was slightly decreased,which was significantly decreased in comparison to Luo Han Guo intervention group at the end of training(P<0.05).The Luo Han Guo intervention group also found a significant difference on IgA before training and after training(P<0.01).(3)No significant difference on CD3 and CD8 was observed among the three groups(P>0.05).In terms of the CD4 levels,the training group was significantly lower than Luo Han Guo intervention group at the end of the training(P<0.05).The CD3 before training was reduced compared with that at the middle of the training(P<0.05),while CD4 showed with a rising followed by decreasing trend.In the Luo Han Guo intervention group,CD3 was rather stable and CD4 was increased with the increase of training time.Both the training group and Luo Han Guo intervention group showed a decreased trend regarding CD8,failing to reach a statistical significance(P>0.05).Conclusion:(1)high intensity training for four weeks can significantly reduce NEU in peripheral blood and decrease IgM,CD3 and CD4,as well as increase the CD4/CD8 ratio,so as to inhibit immunity function;(2)effective components from Luo Han Guo can remarkably increase NEU in peripheral blood and elevate IgG,IgA,IgM and CD4 in T lymphocyte subsets,contributing to the promotion of immunity function,maintain the dynamic balance of immune system and relieve immunosuppression caused by high intensity training.