| Background Living standards have improved steadily in recent years,and the number of acne sufferers is also on the rise.According to epidemiological survey,more than 633 million people in the world have suffered from acne.The significant social health care costs and the impact of personal physical and mental health can not be ignored.More and more studies have shown that the stability of skin microbiota plays an important role in the occurrence and development of acne.Objective High throughput sequencing was used to investigate the composition of bacterial and fungal communities in non lesional areas of acne vulgaris,Objective to evaluate the skin barrier function of patients with acne by measuring skin physiological parameters,and to explore the correlation between skin barrier function and skin microorganisms,as well as the relationship between acne diseases.Methods From 500 volunteers,115 patients aged 18-35 years with acne and 181 healthy people were selected.The skin physiological parameters instrument was used to measure the water content of cuticle,the amount of water loss,oil content,glossiness,heme,melanin,skin pH and skin elasticity in the non lesional areas of the forehead and cheek of the volunteers.Using high-throughput sequencing method,microbial samples were collected from the forehead and cheek non lesional areas of volunteers,and then total DNA was extracted and detected.Then PCR amplification was carried out for bacteria 16S v3-v4 region and fungus ITS2 region.PE library and lllumina sequencing were constructed.Finally,biological information was analyzed.Results A total of 72170860 optimized sequences were obtained from 16S v3-v4 region,and 5761739 sequences were obtained from ITS2 region of fungi.Skin physiological parameters we found that the water content of the cuticle of the forehead in acne patients was significantly lower than that in healthy people(P<0.05).The oil content in the cheek and forehead of acne patients was higher than that of healthy people,and the forehead oil content was statistically different(P<0.01).The melanin content of healthy people was generally higher than that of acne patients,and the melanin content of forehead had statistical difference(P<0.05).The skin elasticity of acne patients was lower than that of healthy people,and the difference of cheek elasticity was statistically significant(P<0.05).The skin compactness of acne patients was lower than that of healthy people,and the cheek had statistical difference(P<0.05).Among them,the difference in transcutaneous water loss(TEWL)and skin PH was not statistically significant.The diversity and richness of bacterial communities in acne population is lower than that in healthy population,and the difference is not statistically significant.At the genus level,Corynebacterium was significantly lower than the healthy group(P<0.01),norank-fNeisseriaceae,Streptococcus,and Neisseria were significantly lower than the healthy group,with statistical differences(P<0.05).The diversity and abundance of fungal communities were greater than those of healthy people,and the difference was statistically significant(P<0.05).There are 6 species with significant differences in the table,namely Malassezia(Malassezia),Trichosporum,unclassified-k-Fungi,unclassified-p-Ascomycota,unclassified-o-Hypocreales and Fusarium.Conclusion This study determined the changes in the skin condition and microbial community structure in the non-lesional area of acne,and determined that the diversity of the microbial population on the skin surface is closely related to the skin barrier function.We need to further understand skin microecology from the perspective of skin barrier.Facial skin microecological disorders and skin condition disorders are the key to the occurrence and development of acne.This research can provide new directions and new research points for acne prevention and care for acne-prone skin.It can also provide a strong argument for the relationship between skin microecological imbalance and acne. |
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