Association Between Polymorphism Of The Molecular Markers Mirna-132 And PNPLA3 Gene And Type 2 Diabetes Mellitus

Objective: T2 DM is caused by both genetic and environmental factors and is closely related to nonalcoholic fatty liver disease(NAFLD).Early warning and timely intervention of T2 DM and its risk factors for NAFLD are of great significance for delaying disease progression.Recent studies have shown that certain cytokines and genetic markers play an important role in the prediction and diagnosis of diabetes and its high risk population.Therefore,from the perspective of epigenetics and genetics,this study will study some molecular markers related to NAFLD,a risk factor of T2 DM,and the occurrence of T2 DM,and discuss the molecular mechanism of diabetes.Subjects: The study was divided into two parts.The first part was to study the association between mi RNA in peripheral blood and non-alcoholic fatty liver disease in NALFD population.A total of 274 subjects were included and divided into 4subgroups: 67 healthy controls,only 73 with NAFLD,68 with T2 DM,and 66 with both NAFLD and T2 DM.The second part discusses the association between PNPLA3 gene polymorphism and diabetes mellitus.A total of 6,821 people were included in the study.There were 3412 patients in the normal control group and 3409 patients in the T2 DM group.Methods: In first part,the relative expression of nafled-related mi RNA in serum was determined by fluorescence quantitative PCR,and the relationship between the serum content and NAFLD and nafled-related clinical indicators was analyzed.In the second part,the genotype of the T2 DM susceptibility gene PNPLA3 rs738409 was identified and its relationship with T2 DM and its related metabolic indexes was analyzed.Results: After adjusting for confounding factors in the first part,serum mir-132 level in the non-t2 dm group was positively correlated with the risk of NAFLD(OR= 3,082,P = 0.039),serum ALT,TG,FPG,gh-gt,and APOE were positively correlated with mir-132.In the second part,the results showed the minority allele G of rs738409 was positively correlated with the incidence of T2DM(OR=1.1,P < 0.001).And risk allele G was negatively correlated with plasma TG levels in both the general population and the normal control population(P < 0.05).Conclusion: The first part of the results suggested that serum mi R-132 may be associated with nonalcoholic fatty liver disease,while the second part suggested an 11%increased risk of type 2 diabetes in those with rs738409 allele G.