Research On Logic Amplification Model Based On Ion Induction And Chain Replacement Technology

Since the British Computer mathematician Turing put forward the computer model Turing machine in 1936,the computer has been developing rapidly.It has become one of the three major scientific revolutions in twentieth Century,and has greatly promoted the development and progress of the society.But with the rapid development of society and technology in twenty-first Century,the complexity of integrated circuits,the ultra-high speed computing power and the storage capacity of silicon wafer is facing bottlenecks,which makes the development of traditional computers encounter unprecedented challenges,and is getting closer to the limit that their technology can achieve,so,the scientists have been looking for reliable alternatives,such as artificial artificial neural network computer,quantum computer,molecular computing,protein calculation and so on,among them,the DNA computer has aroused widespread concern in biology and other sectors,its unique ultra-miniaturization structure makes DNA computer has a large storage capacity,high speed and the appearance of miniaturization,and have been used to solve various complicated problems in the field of biology,For example,NP-complicated problems and SAT problems in Applied Mathematics,drug screening and rapid detection in the field of Biochemistry,rapid detection in Environmental Science.The most notable part is the strong storage capacity of DNA.In 2013,the Goldman team of the European Institute of bioinformatics successfully stored the information of 2.2PB into one gram of DNA.By July 2016,University of Washington and Microsoft announced that their research storage has reached 200MB.We believe that DNA computing can play more powerful functions in the future.Firstly,this paper briefly describes the research background,significance and the research status at home and abroad of the study of computational biology.Secondly,the research techniques and principles used in biocomputing research are introduced in detail,including DNA chain replacement technology,fluorescence labeling technology and DNA structure transformation driven by ions.In addition,the detection methods used in the study are described in detail,including fluorescence detection,polyacrylamide gel electrophoresis and so on.Finally,by summarizing these techniques and methods,and inspiring them from these technologies and methods,the following logical gates are constructed.The specific work content and arrangement of this article are as follows:(1)We mainly expounds the research background,significance and research status of DNA computing and logic gates,and demonstrates the importance of logic gate research for biological computing.Through this chapter,we can fully understand the important role of this research topic,and also show the development and application prospects of DNA computing.(2)We mainly summarize the research methods and techniques about this research used.It mai nly includes the nanotechnology used in the design and construction of logic gates(fluoresce nt labeling technique,DNA strand replacement technique,etc.)and the experimental technolog y(polyacrylamide gel electrophoresis)in the process of verification of the results,to makes more people have some understanding of some important nanotechnology and detection meth ods,and makes a good foreshadowing for the construction of logic gates in the future.(3)We introduces a bidirectional mercury silver detector and a DNA two-stage coupled logic cycle and amplification model,which is constructed by using the specific binding of mercur y and silver ions to specific base pairs.The model not only can detect mercury and silver io ns quickly,highly sensitively and specifically,but also can be constructed into two "OR" logi c gates and a two-stage "AND" logic gate,which is of great application value.(4)We mainly introduces two DNA logic gates using graphene oxide as the signal platform.The model is designed to use the special optical and chemical properties of graphene oxide to quickly and quickly detect the results of the DNA logic gate.Besides,this model can realize the detection of trace DNA by signal amplification strategy of target catalytic hairpin assembly.(5)We introduces two optical driving logic gate and a chain replacement cycle amplification model.We aim to control the opening and closing of logic gates by using the special optical characteristics of MGCB,and realize the recycling of target DNA through two logical gates before and after cascading,so as to achieve the function of signal amplification.This model has a very high DNA detection generality.(6)We have made a summary and Prospect of this subject,including the innovation points,adv antages and disadvantages of the subject and the subsequent experiment optimization.DNA co mputing prospect is huge,and the applicability is high.We expect the researchers to put theDNA into practical use and be widely used in monitoring and improving physical health.