The Intervention Of Jianpi Huatan Quyu Recipe On Liver Lipid Metabolism And The Expression Of AQP3, AQP4 And P-CREB In AS Bama Pigs With Spleen Deficiency And Phlegm Turbidity

Purpose:Established an animal model of hyperlipidemia in Bama miniature pigs,which caused by irregular of diet and fed too much high-fat-diet.These Spleen phlegm pigs were used by a therapeutic method of Invigorate the strking,remove phlegm and remove blood legistation,and which was divided into these groups of “the whole decoction”,“benefiting Q i”,“clearing phlegm and benefiting Q i”,“promoting blood circulation to remove blood stasis”and the group of using simvastin.After using these medicine,these pigs were examined of their situation of cells of liver from destroyed to recovered,and their situation of the express of AQP3,AQP4 and p-CREB from the beginning of using medicine to the finish of using medicine,so that we can know the best group of these pigs.Material and method:The number of 47 SPF grade Bama miniature pigs were selected in this experiment.First,these pigs were fed by ordinary fodder in two weeks to fit the environment of the experiment.After two weeks,other 42 pigs were equally divided into seven animal models of high-fat-diet,except five pigs of blank control group.These 42 pigs were formed Spleen phlegm through by irregular of diet to built the six animal models.After 8 week s,except model control group,other 5 groups were fed by medicine of this experiment once a day,which were continued for 12 weeks.12 weeks later,their tissue of liver were taken from their body to test physiological index: 1.To text their level of TC,TG,LDL-C,HDL-C in their livers;2.HE staining microscopic observation of liver histopathological changes;3.Western blotting was used to detect expression of AQP3,AQP4 and p-CREB;4.All data in this experiment were statistically analyzed using by SPSS 21.0 software.The results were expressed as mean ± standard deviation,and one-way ANOVA was used for comparsion between groups.(P <0.05 indicates statistical significance,P<0.01 indicates significant difference)Results: 1.These all groups of using medicine can reduce the level of TC,TG,LDL-C,and increase the level of HDL-C.Among them,the group of“ clearing phlegm and benefitting Q i”is the best group of reducing the level of TC,the group of“the whole decoction”is the best group of reducing the level of TG,the group of“benefitting Q i”is the best group of reducing the level of LDL-C,the group of“clearing phlegm and benefitting Q i”is the best group of increasing the level of HDL-C.The mean±standard deviation of results are less than 0.05 or 0.01,so the results have statistical significance.2.These all group of using medicine can repair the damage of tissue and cells in liver,and which can reduce the fat in liver,and the best group of it is the“clearing phlegm and benefitting Q i”.The group of western medicine is also can repair the damage of tissue and cells in liver,and can reduce the fat in liver,but it has side-effects.3.These all group of using medicine can reduce the expression of AQP3,AQP4 and p-CREB,which the best group is the“clearing phlegm and benefitting Q i”,and the group“benefitting Q i”has no effect to reduce the expression of p-CREB.Conclusion: 1.The best group to reduce the level of TC in liver is the“clearing phlegm and benefitting Q i”group,the best group to reduce the level of TG in liver is the“the whole decoction” group,the best group to reduce the level of LDL-C in liver is the“benefitting Qi”group,the best group to increase the level of HDL-C in liver is the“clearing phlegm and benefitting Qi”.2.The best group to repair the tissue and cells in liver and reduce the fat in liver is the“clearing phlegm and benefitting Qi”.3.The best group to reduce the expression of AQP3,AQP4 and p-CREB is the“clearing phlegm and benefitting Qi”.