The Effect Of The Method Of Invigorating The Kidney And Adjusting The Bubble Cycle On The Expression Of AMHR?receptor/Smad4 Protein In The Ovaries Of Polycystic Ovary Model Rats

Objective: To study the regulation of Bushen-follicle recuperation therapy on the expression of AMH type ? receptor(AMHR ?)Protein and Smad4 expression in PCO rat models,to investigate the mechanism of Bushen-follicle recuperation therapy for polycystic ovary syndrome(PCOS).Methods: Ten of 110 female SD rats were randomly selected as normal group,the remained 100 rats were divided into model group,positive control group,A-xiaoyao pulvis high-,and low-dose groups,B-sanzi decoction high-,and low-dose groups,A+B-xiaoyao pulvis+sanzi decoction group,with 10 rats in each group.The increment of body mass was statistically analyzed.Ovarian organ coefficient were measured.The pathological morphology of ovarian tissue was observed by HE staining.AMHR ? and Smad4 protein expression of PCO rat ovarian were detected by immunohistochemistry method.Results: Body mass increment: Compared with the normal group,the body mass increment of PCO model group was increased significantly(P<0.01).Compared with the model group,the body mass increment of A low group was increased,B high group,B low group and positive control group was reduced(P<0.05).Ovarian coefficient: Compared with the normal group,the ovarian coefficient of PCO model group was increased significantly(P<0.01).Compared with the model group,the ovarian coefficient of A low group reduced(P<0.05),and that of A high group,B high group,A low group,B low group,A high group and B low group and the positive control group reduced significantly(P<0.01).AMHR ? protein expression: Compared with normal group,AMHR ? protein expression of the PCO model group rats ovarian tissue were increased significantly(P<0.01).Compared with model group,AMHR ? protein expression of A low dose group were reduced(P<0.05),more than the treatment group and positive control group of AMHR ? protein expression were reduced significantly(P <0.01).Comparison between treatment group: Compared with group B high,AMHR ? protein expression of A low group and B low group rat ovarian tissue were increased significantly(P<0.01),AMHR ? protein expression of A high group rat ovarian tissue were increased(P < 0.05),AMHR ? protein expression of A high B high group were reduced significantly(P < 0.01).Compared with B low group,AMHR? protein expression of B high group,A + B cycle group and positive control group significantly were reduced(P< 0.01).Compared with A low B low group,AMH R ? protein expression of A(low and high)group,B low group,A high B low group were increased significantly(P<0.01).Compared with A low B high group,AMHR ?protein expression of A(low and high)group,B low group were increased significantly(P<0.01),AMH R ? protein expression of A high B high group were reduced significantly(P<0.01),AMHR ? protein expression of A high B low group were reduced(P<0.05).Compared with A high B high group,AMHR ? protein expression of A(low and high)group and B(low and high)group,A low B high,A high B low group were increased significantly(P<0.01).AMH R ? protein expression of comparison between A+B cycle groups: A high B high < A low B high group < A high B low group.Smad4 protein expression: compared with the normal group,Smad4 protein expression of the PCO model group was significantly increased(P<0.01).Compared with the model group,the Smad4 protein expression of A low group was reduced(P<0.05),and those of A high,B high and A+B cycle groups were reduced significantly(P<0.01),but there was no significant difference among A group,B group and A+B cycle groups(P>0.05).Conclusion:The regulation of Bushen-follice recuperation therapy significantly reduce the increment of body mass.The ovarian coefficient of PCO model rats was significantly reduced.The histopathological morphology of ovary was improved.And the AMH R? and Smad4 protein expression of PCO model rats were obviously reduced.The regulation of Bushen-follice recuperation therapy may affect follicle recruitment and selection by inhibiting the AMH signaling pathway,promote follicle development and maturation,and thereby restore ovarian morphology andfunction.