Follicular Development And Ovulation Disorders, Kidney And Blood Stasis Animal Model Studies

OBJECTIVE To find an animal model that helps to evaluate the effect of Zichong particles whose function is to tonify kidney, dissolve blood stasis, promote follicular development and ovulation. METHLDS: To inspect the markers such as hemorheology, estradiol, progesterone, the exponent of ovary and uterus, the number of growing follicle,ripe follicle, atresic follicle and luteum, the depth of endometrium, the number of glands of kidney deficiency model results from hydroxyurea, blood stasis model results from cortisol and epinephrine . the blockade of ovulation animal model with kidney deficiency and blood stasis results from sigle progesterone contraceptive DMPA,LNG and Traditional Chinese medicine. Results: The ovary exponent and the number of follicle of the kidney deficiency model results from hydroxyurea reduced, but the model came out without blood stasis; The blood stasis model results from cortisol and epinephrine had abnormal hemorheology, but without blockade of follicular development and ovulation; The models result from progesterone contraceptive and Traditional Chinese medicine had different degrees of abnormal hemorheology , increasing growing follicle and atresic follicle, reduced luteum and glands, low progesterone level, which imply that progesterone has the function of making the rats' hemorheology abnormal and ovulation inhibition. Conclusion: The ovulation inhibition and blood stasis animal model results from progesterone can help to evaluate the effect of Zichong particle and other Traditional Chinese medicine which can tonify kidney, dissolve blood stasis and promote ovulation.