Study On The Intervention Effect Of Acupuncture On Patients With Acute Cerebral Infarction In Basal Ganglia Region Based On Metabonomics

Objective:Taking ACI patients in the basal ganglia area as the research object,combined with UPLC-Q-TOF/MS technology,non-targeted metabonomics studies were performed on serum biological samples to find differential biomarkers of ACI in the basal ganglia area and study the intervention effects of acupuncture.Method:1.According to the inclusion and exclusion criteria,35 patients(5 patients with abscission)with ACI in basal ganglia were selected as the disease group,collected the patient’s blood samples before treatment,processed them within 2 hours,separated the serum,and then placed the cryotube containing with the serum sample in a-80℃ refrigerator for sealing.At the same time collected 25 healthy human serum.After the samples were collected,taken 100 μL of serum from each sample,added 300 μL of acetonitrile(1: 3 volume ratio),vortexed and mixed for 1 min,iced and watered bath for 10 min,centrifuged at 13000 rpm/min at 4°C for 15 min,removed 200μL of supernatant and then put into the sample vial for UPLC-Q-TOF/MS liquid quality analysis.First,Masslynx software was used to reduce the dimension of the original data and acquire mass spectrometry matrix information,to find out the potential discriminant variables,and then the data was normalized and exported,after 80% data modification,imported into SIMCA-P12.0,constructing unsupervised principal component analysis(PCA)and supervised partial partial least squares-discriminant analysis(PLS-DA)model,preliminary screening of potential markers.At the same time,R2 X value,R2 Y value and Q2 value were used to evaluate the model.the closer the above values was to 1,indicating that the more stable and reliable the model was established.Differential variables were screened out in the PLS-DA model(VIP>1).Use the m/z value of the significantly different metabolites to search for possible substances in the HMDB and Chemspider database,and further use the detected differential metabolic small molecules,through MS/MS analysis,metabolite database fragment information and literature fragments Information to confirm the selected candidate markers as potential biomarkers for ACI in the basal ganglia area.2.ACI patients in the basal ganglia area were divided into 20 cases in the observation group and 10 cases in the control group.The control group was given routine treatment.The observation group was treated with acupuncture on the basis of the control group.After 2 weeks of treatment,the patient’s blood samples were collected.After the treatment in the above manner,the patient’s serum before treatment was compared and analyzed to observe the changes in the content of the above biomarkers before and after treatment.Result:1.Screening serum differential biomarkers of ACI patients in basal ganglia based on metabonomics: through comparative analysis with healthy groups,PCA and PLS-DA models were successfully constructed,and finally seven differential biomarkers were selected-proline,methionine,tryptophan,pyroglutamic acid,Lyso PC,inositol,phenylalanine.Compared with the healthy group,the content of Lyso PC in the disease group was lower than that in the healthy group,with statistical significance(P<0.01),and the content of the remaining markers was higher than that in the healthy group,with statistical significance(P<0.05,P<0.01).2.Study on the intervention effect of acupuncture on patients with Acute cerebral infarction in basal ganglia region based on metabonomics:(1)After 2 weeks of treatment,compared with before treatment,the control group had three biomarker content callbacks after treatment,namely methionine,pyroglutamic acid,and phenylalanine,and the observation group had five biomarker content callbacks after treatment,namely methionine,tryptophan,pyroglutamic acid,inositol,and phenylalanine,the contents decreased compared with that before treatment,and all with statistical significance(P<0.05,P<0.01);(2)After 2 weeks of treatment,compared with before treatment,the content of proline,tryptophan and inositol in the control group was lower than that before treatment,and the content of Lyso PC was higher than before treatment,but no statistical significance;the content of proline after treatment in the observation group was higher than before treatment,but no statistical significance,the content of Lyso PC was lower than before treatment,andd with statistical significance(P<0.01).Conclusion:1.Serum metabonomics suggests that there is a serious abnormality in amino acid metabolism and phospholipid metabolism after ACI in the basal ganglia.2.After acupuncture intervention,the number of regulated metabolites is greater and the metabolic pathways involved are broader.Regulating the metabolism of amino acids and phospholipids to make the body’s disordered metabolism more normal,it may be an important acupuncture treatment for acute cerebral infarction.