Resveratrol Appears To Protect Against Oxidative Stress Of Testis In Rats By PAEs

Objective: To study,the oxidative stress injury model of SD rats was established by intragastric administration of MIXPs,and the protective effect and mechanism of resveratrol on oxidative stress injury.Methods: 48 Male SD rats were randomly divided into 4 groups(n=12).Group A: blank control group;Group B:model group;Group C: Resveratrol(RES)group,Group D: MIXPs+RES group.The observation indexes of each group included: 1.General condition of rats(mental state,hair,diet,exercise,death,weight,etc.);2.Organ coefficient of testis;3.HE staining was used for observing the morphological structures of testicular tissue;4.Detection of serum hepatorenal function by biochemical analyzer;5.Serum T,LH and FSH concentrations were measured by ELISA;6.Serum SOD,GSH-Px,CAT and MDA were quantified by enzyme linked immunosorbent assay;7.The expression of HO-1and HSP-60 in testis.Results: 1.Compared with the blank group,the MIXPs exposure model was successfully established,which showed that the weight and testis weight of rats in the exposure group decreased significantly(P<0.01),and the testis coefficient had no significant difference(P>0.05).The outline of seminiferous tubules was slightly damaged,and the cells were arranged irregularly.It can be seen that the seminiferous cells fell off and there was no sperm in the lumen.The content of T,FSH decreased significantly(P<0.01).The serum SOD,GSH-Px,CAT and MDA.The expression of HO-1 and HSP-60 in testis decreased significantly(P<0.01).2.Resveratrol had no significant toxic effect on rats,which showed that there was no significant difference in body weight,testicular weight and organ coefficient between resveratrol group and blank group(P>0.05),there was no significant difference in testicular histopathology.There was no significant difference in serum T,LH and FSH levels(P>0.05),There was no significant difference in serum SOD,GSH Px,CAT and MDA(P>0.05).There was no significant difference in testicular tissue.There was no significant difference in the expression of HO-1 and HSP-60(P>0.05).3.Resveratrol intervention can prevent oxidative stress injury caused by MIXPs.The results showed that: there was no significant difference in body weight and testis weight between the intervention group and the blank group,which was significantly higher than that of the model group(P<0.01).The contour of the convoluted fine tubule was complete,the arrangement of spermatogenic cells at all levels was slightly disordered,no exfoliation of spermatids,and a smallnumber of spermatozoa were seen,which was obviously superior to the model group.serum T,FSH content was higher than that of the model group(P<0.01).The level of LH was lower than that of model group(P < 0.01).The levels of SOD,GSH-Px and cat in serum were significantly higher than those in the model group(P<0.01),MDA was significantly lower(P<0.01)and the expression of HO-1 and HSP-60 in testis was significantly higher than that in the model group(P<0.01).Conclusions: 1.The model of oxidative stress injury of rat reproductive system can be established by intragastric administration of MIXPs.2.Resveratrol has no obvious toxic and side effects on rat reproductive system through continuous intragastric administration.3.Resveratrol can reduce the damage of MIXPs on rat reproductive system,and its mechanism may be related to regulating hormone level in rats and inhibiting testis group oxidative stress in the fabric is related,which may have a certain preventive effect on oxidative stress injury of reproductive system caused by MIXPs.