| Objective: to study the effect of cleanser use on skin microbiome in children with atopic dermatitis and its correlation with AD.Methods: collected in October 2017 to July 2018 in our department during the outpatient clinic or ward 1.5-16 years old children hospitalized with moderate to severe AD patients,according to the bath there is no use cleaner,divided into no-cleaner and cleaner group,record its clinic for the first time SCORAD scores and related clinical data,multipoint sampling to return the skin lesions and normal skin specimens.After that,patients in both groups were treated in accordance with the relevant guidelines for AD,while those in the no-cleaner group had their bathing habits corrected.Follow-up was conducted after 4 weeks of treatment to re-evaluate the SCORAD score and re-collect the skin microbial samples at the original site.Meanwhile,skin microbiological specimens of patients with skin surgery at the same age were collected as the normal controlgroup.All skin microorganism samples were sequenced by 16s-rDNA,and the sequencing results were analyzed by ?-diversity,?-diversity,species classification,univariate statistical comparisons and LEfSe analysis.Results: a total of 49 children with AD were collected,including 37 in the no-cleaner group,12 in the cleaner group,and 20 in the normal control group.Sixty-nine skin microflora specimens were collected at the initial diagnosis in the no-cleaner group.Among the 37 patients,14 were followed up after 4 weeks of treatment,and 36 skin microflora specimens were collected after treatment.35 skin bacterial flora samples were collected from patients in the cleaner group at the first diagnosis,and 4patients were followed up after 4 weeks of treatment,and 12 skin bacterial flora samples were collected after treatment.A total of 31 samples of skin microflora were collected from 20 people in the normal control group.There was no significant difference in SCORAD score between the two groups after treatment.Between the two groups of AD patients and normal controls on the part of the ?-diversity index and ?-diversity analysis,univariate statistical comparisons and LEfSe analysis shows that the biggest difference between the three groups are abundance of the g_Staphylococcus.The g_Staphylococcusis a marked increase in the total abundance in patients with AD,but the abundance ofStaphylococcus_hominis in normal control was higher than AD patients.There was no statistical differences in SCORAD scores between no-cleaner group and cleaner group after 4 weeks after treatment.There was only individual differences in ?-diversity index between the two groups of patients,and there was no difference in ?-diversity analysis,univariate statistical comparisons and LEfSe analysis shows there was no difference in the abundance of g_Staphylococcusis between the two groups of AD patients.The main differences between these two groups of AD patients is abundance of g_Propionibacterium.The results showed that the g_Propionibacterium abundance was low in the no-cleaner group,and it increased after intervention.However,there was no significant difference in the g_Propionibacterium abundance before and after treatment in cleaner group.Conclusion: the main difference in skin microbiome between AD patients and normal controls was from g_Staphylococcusis,while the main difference in skin microecology between AD patients and normal controls was from g_Propionibacterium.By comparing the changes of g_Propionibacterium abundance before and after treatment between these two groups,we speculated that the use of bath cleaners may affect the skin microbiome of AD patients to some extent,but its long-term effects on thecondition of AD and skin microbiome,as well as whether the use of cleaners can bring actual benefits to patients still need to be further studied. |
PDF Full Text Request