Study On Metabonomic Of Essential Hypertension Yin Deficiency And Yang Excess Syndrome Based On UHPLC/Q-TOF-MS

Objective:The metabolites of hypertension syndrome of Yin deficiency and Yang hyperactivity were observed by means of metabolomics,the serum metabolites of hypertension patients with Yin deficiency and Yang hyperactivity were compared with those of healthy people,and the potential biomarkers of hypertension syndrome of Yin deficiency and Yang hyperactivity were searched,which further provided the basis for modern TCM syndrome differentiationMethods:According to the diagnostic criteria and inclusion criteria,41 cases of hypertensive patients with Yin deficiency and Yang hyperactivity syndrome and 30 cases of healthy control group were selected.We extract the subjects on an empty stomach after 12 hours of venous blood,blood routine and biochemical examination after collecting basic data,and then kept in the refrigerator-80?,after being collected samples,sample preparation,by high performance liquid chromatography(HPLC)with quadrupole time-of-flight mass spectrometry technology(UHPLC-Q-TOF/MS)analysis of serum metabolic spectrum characteristics of all subjects.The metabolomics data were analyzed by multivariate statistical method to extract the differential metabolites.Results:(1)Comparison of basic information:there was no statistical difference in gender and age between the two groups(P>0.05).There was no significant difference between the two groups in blood routine and some major indexes of blood biochemistry(P<0.05).(2)By using principal component analysis(PCA)and orthogonal partial least-squares discriminant analysis method(OPLS-DA)for data analysis,can be found that hypertension group and healthy control group,two groups of serum metabolites samples there is obvious difference,38 different metabolites were identified as potential biomarkers.The significant difference metabolites(P<0.05)were:Acetyl carnitine,L-glutamic acid,L-tryptophan,mustard acid amide,lactic acid,DL-indole-3-palmitoyl carnitine,nerve sheath phospholipids,ornithine,L-glutamic acid,anthranilic acid,ammonia ketone of goose deoxycholic acid sodium,alpha isovaleric acid,hydroxy palmitic acid,allantoin,10-26 alkyl acid,linoleic acid cis epoxide,pentadecane acid and ethylenediamine tetraacetic acid,valeric acid,goose deoxycholic acid salt,cis-11,14 and 20 carbon diene acid,hydroxy lauric acid,oxygen generation of adipic acid,myristic acid,taurine,DL-4-hydroxy-3-methyl phenyl lactic acid,benzoic acid,L-gulonic acid-gamma-lactone,octadecenoic acid.The difference metabolites(0.05<P<0.1)were:1-stearyl n-sn-glycero-3-phosphoylcholine(18:0 lyso PC),18:1 lysogenic lecithin,thioetheramide-pc,ceramide,n-dodecanoic acid,epoxy oleic acid,alanine,dihydroxyacetone,and 1-proline.These differential metabolites may be potential biomarkers of hypertension syndrome of Yin deficiency and Yang hyperactivity.Research conclusion:(1)The serum metabolites of patients with hypertension with Yin deficiency and Yang hyperactivity syndrome are indeed different from those of healthy people,which is helpful for the differential diagnosis between patients with hypertension with Yin deficiency and Yang hyperactivity syndrome and healthy people.(2)A total of 38 different metabolites including significant difference metabolites and different metabolites were found.These differential metabolites may be potential biomarkers for hypertension with Yin deficiency and Yang hyperactivity.(3)Compared with the results of previous metabolomics studies on the deficiency syndrome of Yin and Yang in hypertension,the same difference metabolite is tryptophan.Other metabolites,such as acetylcarnitine,ornithine,sphingomyelin,sodium glycyrrhizin,may become the differentiation points of Yin deficiency Yang hyperactivity syndrome and Yin and Yang deficiency syndrome in hypertension.This may provide some objective basis for the modern differentiation of TCM.