The Efficacy And Mechanism Of Needle Knife Treatment Of Secondary Biceps With Long Head Tendinitis Rats

Objective:This topic aims to make the biceps short head injury secondary to the biceps tendon inflammation model,to observe the biceps short head injury after the biceps long head tendon of the change of the morphology and the related immune factors,explore the biceps short head injury in the late development of biceps tendinitis experimental basis,to provide human anatomical bowstring mechanics system theory in acupotomology laboratory research foundation.Explore the needle knife to subsequent biceps tendinitis treatment curative effect and its mechanism,for the needle knife provide theoretical basis for the clinical treatment of chronic soft tissue injuries.Methods:After 1 week of adaptive feeding of 50 rats,50 rats were randomly divided into blank group,model 1 group,model 2 group,electroneedle group,needle group,10 per group.The total duration of the experiment was 8 weeks,the first 4 weeks of the model group,acupuncture group and needle group rats unified modeling,from the5 th week of treatment.Blank group,conventional feeding,do not do any treatment,the rest of the group are given the "Type Ⅰ collagenase combined running table movement" method to establish a secondary biceps tendonitis model.Step 1: Collagenase injection.After putting on a special rat suit,the front of the rat’s shoulder is skinned.The rat is then fixed to the rat plate and injected with20ul(0.015 mg/ul)collagen solution into the tendon of the lower biceps.Step 2: Centrifugation motion.Place the rat on the electric running platform and gradually increase the speed to 17m/min for a 60min-17 o downhill run.Model 1 group after the start of the mold,model 2 group after the start of the mold 4 weeks,the fifth week of the electric needle group began electro-needle treatment,once a day,a total of treatment in January.The specific operation method is to fix the rat on the mouse table,when fixed action to be light,as little as possible to the rat stimulation.Referring to the human body’s acuity standard,select the left side of the rat "shoulder" and "arm" hole,with a one-time 1 inch acupuncture needle into about 5 mm,with Han’s acuity nerve stimulator power,electric needle frequency of 2/100 HZ of dense waves,current strength of not more than 2 m A,to local micro-vibration and animals do not hissase,each 15,10 days for a total of 2 days of treatment.Needle knife group in the fifth week to start needle treatment,in the anaesthetic state will be fixed rats,the rat shoulder touch at the spot at the spot,according to the needle knife four-step procedure for the needle,the knife line and the rat limb long axis direction is consistent,in the outside of the protrusion to insert cutting 1 knife,range 0.1-0.2cm,after the needle press 1 min,to prevent bleeding,treatment 1 time.Week 8.The general condition,behavioral characteristics,and HE staining patterns of the biceps in each group of rats were recorded and analyzed,and the expression level of the inflammatory-related factors IL-1β,IL-6 was detected by ELISA,and the expression level of the mechanical related factor MGF was detected.Experimental data processing is analyzed using SPSS 22.0 statistical software.Results:1.behavior: the wilderness,experimental results show that the normal control group model as compared to the group of rats significantly reduced the total activity away,there is a very significant statistical differences(P<0.01);and model group,compared to the current pin and pin knife set of mouse total activity from the contrast between groups have a very significant statistical differences(P<0.01).Direct observation found that the model group of rats and normal control rats than in the 神,posture,the hairs are showing a sick.Pin tool set,the Pin group rats and normal control rats than in the manner,posture,hair,there are no significant differences.2.Organizational morphology: Normal Rats And The biceps long head muscle tissue fibers of the muscle are Linear or microwave wave-shaped,parallel arranged collagen fibers;the more evenly and neatly around the muscle fibers;visible between the small capillary blood,rare inflammatory cells.But with the biceps long head tendon inflammation of the mouse with the biceps long head muscle tissue structures of the muscle fiber twist,arrange messy,wavy-shaped;the Convention on the Rights of the marked increase in the core,distribution of collagen Fiber Arrangement;promiscuous;a large number of visible capillary hyperplasia and inflammatory cells to soak.The Pin and Pin knife after the intervention,and the biceps long head muscle tissue,inflammatory cells soak reduced,muscle fibers and collagen fiber structure is not subject to significant damage.3.IL-1 β level,IL-6 level,MGF level:model group of rats,organizations IL-1 β level,the level of IL-6,MGF levels are higher than normal contrast group,there is a very significant statistical differences(P<0.01);the pin and pin knife set of mouse muscle organization IL-1 β level,the level of IL-6,MGF levels are below the model group of rats,the contrast between groups have a very significant statistical differences(P<0.01);pin knife set of mouse muscle organization IL-1 β level,the level of IL-6,MGF levels are below the pin group,there is a very significant statistical differences(P<0.01).Conclusion:1.Using the "method of collagenase injection combined with centrifugal movement" to make secondary biceps tendinitis rats model success possible,needle knife therapy and cupping treatment secondary biceps tendinitis and effective,and the curative effect is superior in terms of needle knife.2.The needle knife and cupping treatment mechanism and related to the regulation of inflammatory factors and mechanical factors,needle knife for more effective regulation of inflammatory factors and mechanical factors.3.The needle knife therapy secondary biceps oil needle knife and the mechanism of tendinitis biological force regulating the release of soft tissue.