Diagnostic Value Of Heat Shock Protein 90? And Squamous Cell Carcinoma Antigen In Detection Of Cervical Cancer

Objective:To ascertain plasma levels of heat shock protein 90??HSP90??and squamous cell carcinoma antigen?SCC-Ag?and their diagnostic potential in cervical cancer.Methods:In a cross-sectional study,patients'cervical tissue samples were screened for high risk?HR?human papilloma virus?HPV?DNA and underwent a thinprep-liquid based cytology test?TCT?.Plasma samples were analysed by enzyme-linked immunosorbent assay?ELISA?for HSP90?and SCC-Ag levels.Results:Of the 295 women who underwent screening,75 were healthy controls 75(HR-HPV^-veve TCT^-ve),110 were HR-HPV⁺veve TCT^-veve and 110 were HR-HPV⁺veve TCT^+ve.There were significant differences between levels of HSP90?and SCC-Ag proteins across the patient groups with those positive for cervical cancer having the greatest levels of proteins compared with other groups.For patients with high grade SCC there was a significant correlation between levels of HSP90?and SCC-Ag.HSP90?was positively correlated with the viral load of HR-HPV.The area under the ROC curve for combined HSP90?*SCC-Ag was the largest compared with the single proteins.Using a cut-off value of 16.4 ng/ml to delineate cervical cancer diagnosis,the sensitivity and specificity of HSP90?*SCC-Ag were 90.3%and 95.1%respectively.Conclusion:Plasma HSP90?protein levels correlated well with SCC-Ag levels in patients with cervical cancer and the combination of HSP90?*SCC-Ag may be a useful diagnostic biomarker.The content of HSP90?is closely related to the positive rate of HR-HPV viral load.