The Expression Level Of NMP238 And HSP70 In Human Cervical Squamous Cell Carcinoma Associates With The Radiation Therapy Sensitivity

Objective:To investigate the association of the expression of NMP238 and HSP70 in pre-radiotherapy tissues with the radiation therapy sensitivity in human cervical squamous cell carcinoma.Methods:1. 60 samples from patients with pathologically diagnosed as cervical squamous cancer were collected, as well as 30 normal cervical tissues used as control. These 60 cervical squamous cancer samples were divided into four groups according to their different pathological grades and clinical stages as: low-differentiation, middle-high-differentiation, earlier-phase and middle-late phase. Meanwhile, those 60 cervical squamous cancer samples were also classified into two groups according to the response to RT as radiosensitive(CR) and radioresistant groups(PR).2. Immunohistochemical experiments were performed to detect the expression levels of NMP238 and HSP70 in 60 cervical squamous cancer and 30 normal cervical tissues.3. The expression levels of NMP238 and HSP70 in normal cervical tissues and cervical squamous cancer tissues of various pathological type and stage were compared. The potential association of expression levels with radiation therapy efficiency was also analyzed.Results:1. In cervical squamous cell cancer tissues, the expression levels of NMP238 and HSP70 were significantly higher (p<0.05) than these in normal cervical tissues. The expression positive rate of NMP238 and HSP70 in cervical squamous cell cancer tissues were 85% and 71.7%. However, the expression positive rate of NMP238 and HSP70 in normal cervical tissues were 43.3% and33.3% respectively.2. Neither the expression of NMP238 nor that of HSP70 in cervical squamous cell cancer tissues was significantly associated with their pathological grades (p>0.05).3. The expression of NMP238 and HSP70 in cervical squamous cell cancer tissues were significantly correlated with their clinical stages (p<0.05).4. The expression of NMP238 in cervical squamous cell cancer were positively correlated with that of HSP70 by Pearson correlation analysis (C=0.92,P<0.05).5. 36cases in 60 patients were complete response, whose rate is 60%(36/60); while partial response, no change and progress disease were 24, 40%(24/60); 0and0; respectively.6. In radioresistant groups, NMP238 were generally highly expressed and their overexpression rate were 75%( 18/24), which is significantly higher (p<0.05) than that in radiosensitive groups, whose rateis 47.2%(17/36).7. The overexpression rate of HSP70 in the radiosensitive group was 22.2% (8/36), and it was significantly lower than that in radioresistant groups which was 56.2%(13/24) (P < 0. 05).Conclusions:1. The expression of NMP238 and HSP70 in human cervical squamous cancer tissues were significantly higher than these in normal cervical tissues. There is a correlation between the expression of NMP238 and HSP70 in squamous cell cancer with their clinical stages. It highly suggests that NMP238 and HSP70 play important roles in the tumorgenesity , development and prognosis of cervical squamous cell cancer.2. There is a co-relationship between the expression of NMP238 and HSP70 in squamous cell cancer, which may suggest that their effects were additive.3. The overexpression of NMP238 and HSP70 were associated with tumor radioresistance. It suggests that overexpression of NMP238 and HSP70 can be an useful predictor for the radioresistance in cervical squamous cell carcinoma cancer.