| Objective: To observe the effect of Sinew-regulating and bone-setting manipulation on the inflammation of rabbit knee osteoarthritis model and explore its mechanism.Methods: Twenty-four healthy and clean 6-month-old New Zealand rabbits(half male and half female,2.5 ± 0.5kg in weight)were randomly divided into three groups,with 8 rabbits in each group.The three groups were ZC(normal)group,MX(model)group and SF(Sinew-regulating and bone-setting manipulation)group.The ZC group did not participate in the modeling,and the other two groups used the modified Hulth modeling method to prepare the rabbit model of knee osteoarthritis.The MX group did not intervene and was reared as the ZC group.At the end of modeling and at the end of intervention,behavioral observation was carried out to record and compare the differences of each group.After sampling,knee joint was observed in each group,and cartilage histopathological changes were observed by HE staining.Nitrate reductase method was used to determine the concentration of NO in serum and joint fluid.The concentrations of PGE2,IL-1? beta and TNF-? beta in serum and articular fluid were determined by enzyme-linked immunosorbent assay.Apoptosis of chondrocytes was observed by Tunel terminal fluorescence labeling.Results:(1)Behavioral observation of the knee joint: there was no swelling and deformation of the knee joint in the ZC group,with soft muscles,normal gait and jumping Jump a powerful.In the MX group,the knee joint was enlarged and enlarged,quadriceps femoris muscle tone was increased,and the patchy and strip-like tendons could be touched around the joint.In the limping gait,the right lower limb was weak in weight bearing,and the center of gravity was shifted to the left.After manipulation treatment,muscle tone was reduced,joints were dissipated and gait was improved in SF group.The difference of Lequesne MG score in each group was statistically significant(P<0.05).(2)Gross and histological observation of the genu cartilage: the right tibial plateau and the medial and lateral femoral condyle of the ZC group were smooth and crystal,with a light blue color under natural light.Hyperplasia of the right medial femoral condyle was more obvious than that of the lateral condyle in the MX group,presenting a light yellow.The chondrogenic surface of the medial condyle of the right femur in the SF group presented mild hyperplasia,and the lateral condyle was smooth and smooth.The comparison and pairwise comparison of Mankin scores in the three groups were statistically significant(P<0.05).(3)Nitrate reductase and ELISA were used to determine the contents of NO,PGE2,IL-1?,TNF-? in serum and joint fluid.The results showed that the levels of NO,PGE2,il-1 and TNF-in the serum and joint fluid of the MX group were significantly higher than those of the ZC group and the SF group.The difference between the three groups and pairwise comparison was statistically significant(P<0.05).(4)Chondrocyte apoptosis was observed by Tunel method.Tunel test results showed that: compared with ZC group,the number of chondrocyte ap in MX group and SF group increased significantly,and thoptosise difference was statistically significant(P<0.05).Compared with MX group,the chondrocyte apoptosis index of SF group was significantly reduced(P<0.05).Conclusion:(1)The tendon and bone setting technique can reduce the contents of NO,PGE2,IL-1? and TNF-? in serum and joint fluid of the rabbit model of knee osteoarthritis,and play a therapeutic role in the inflammation of knee osteoarthritis.(2)Tendon and bone setting can inhibit the apoptosis of articular cartilage cells in the model of knee osteoarthritis and delay the degeneration of articular cartilage.(3)Tendon regulation and bone setting can improve the motor function of knee joints in rabbits with knee osteoarthritis. |
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