Qianhu Decoction On The Clinical Efficacy And Mechanism Of Acute Bronchitis In Patients With Phlegm-heat Depression

Research Objectives:This subject comprehensively evaluates the safety and effectiveness of clinical use of Qianhu Decoction in the treatment of patients with acute bronchitis due to phlegm-heat stagnation of lung syndrome;at the same time,it establishes a rat model of acute bronchitis and examines before The mechanism of Hu Tang on acute bronchitis provides a theoretical basis for future treatment options.Research method:1.Clinical study:This study was divided into the treatment group(Qianhutang plus western medicine)and the control group(amoxicillin dispersible tablet,Huifening),and collected the sputum fever with acute bronchitis in the Nanjing Integrated Traditional Chinese And Western Medicine Hospital respiratory medicine clinic from January 2019 to January 2020.60 patients who met the diagnostic criteria of acute bronchitis and phlegm-heat syndrome,of which 28 were in the treatment group(2 out of the group),18 were male,and 10 were female;29 were in the control group(1 in the disconnected group),including 17 male and 12 female.The randomized parallel control method was used to observe the changes in TCM symptom scores(including the frequency of cough attacks before and after treatment,the quality of sputum color and pharyngitis,etc.)before and on the 7th day after treatment,and judge the comprehensive efficacy;The changes of C-reactive protein in the serum of patients were measured before and after to evaluate the clinical efficacy.During the treatment process,the patient's vital signs were closely monitored for any adverse reactions,and blood routine and liver and kidney function levels before and after treatment were taken as safety indicators.2.Animal research:A total of 64 SPF SD rats were used to establish a model of acute bronchitis in rats by fumigation,and they were randomly divided into 4 groups:model group(distilled water 20ml/kg,gavage),positive Drugs Huifeining treatment group(huifeining 10ml/kg,gavage),Qianhu decoction high-dose group(decoction 20ml/kg,gavage),Qianhu decoction low-dose group(decoction 10ml/kg,gavage):16 rats each,and then take 16 normal rats as blank control group(distilled water 20ml/kg,gavage).Dosing was continued for 7 days,once a day.Observe the changes of the rat's respiratory system according to the score table before daily administration.The rats' weights were measured on the 3rd and 7th days after administration,and the orbital blood of the rats was taken to measure the levels of interleukin 2,interleukin 8 and intercellular adhesion molecule 1 after 2 hours of administration.The number and proportion of lymphocytes,neutrophils,and eosinophils in the alveolar lavage fluid were measured with the lotion;the bronchial and lung tissues of rats were dehydrated,sliced,and stained,and the infiltration of inflammatory cells around the bronchi was observed under a microscope.Whether there is exudate in the lumen,changes in alveolar wall interstitial inflammation and alveolar emphysema,whether the bronchi are dilated and so on.Research result:1.Clinical study:TCM symptom scores and serum C-reactive protein levels in the two groups were significantly lower than before treatment(P<0.05),indicating that the two groups were effective;after 1 course of treatment,the treatment group and the control group Compared with the control group,except for the three symptom scores(P>0.05)of pharyngeal itching,lung snoring,and facial redness,there was no statistical significance.The remaining scores and the content of C-reactive protein in the serum were lower in the treatment group than in the control group.Significantly(P<0.05);the overall efficacy analysis of the two groups showed that the total effective rate of the treatment group was 96.43%higher than that of the control group(89.66%)(P<0.05).During the course of treatment,the vital signs of the two groups of patients were stable.There were no obvious abnormalities in blood routine,liver and kidney function before and after treatment,and the patients did not experience other adverse reactions.2.Animal research:Compared with the three groups after treatment,Qianhutang high-dose treatment group can significantly improve the serum inflammatory factor levels in model rats(P<0.05),and Huifeining group and Qianhutang low-dose group also have an improvement effect(P>0.05);the three groups have a significant improvement in the number and proportion of leukocytes in bronchoalveolar lavage fluid of model rats(P<0.05),but the effect intensity of Huifeining treatment group is weaker than that of Qianhu Tang high-dose group;The high-dose treatment group had a better effect on bronchitis cell infiltration in model rats,and the effect was more pronounced after 7 days of administration.The number of bronchitis cell infiltration in the Huifening and Qianhu decoction groups was also reduced.Conclusion:Qianhu Decoction can effectively improve the clinical symptoms of patients with acute bronchitis,phlegm-heat stagnation and lung syndrome,and reduce the C-reactive protein content in patients' serum.Compared with patients treated with western medicine alone,the clinical symptoms are alleviated more significantly.At the same time,Qianhu Decoction can also reduce the levels of inflammatory factors in the serum of acute bronchitis model rats,improve the number and proportion of leukocytes in bronchoalveolar lavage fluid of model rats,and also have a good effect on the infiltration of bronchitis cells in model rats.This topic confirms the safety and effectiveness of Qianhu Decoction in clinical use,and provides a theoretical basis for the clinical use of Qianhu Decoction.