| Objective:Through questionnaires for general information and oral hygiene habits,screening of the neurological scale,periodontal examination and the detection of periodontal pathogens P.g and T.d in patients with moderate-to-severe periodontitis and moderate-to-severe periodontitis with cognitive dysfunction.They were detected to explore the correlation between cognitive dysfunction and periodontal disease.To explore the correlation between moderate and severe periodontitis and cognitive dysfunction,and to provide new ideas for prevention of cognitive dysfunction from the perspective of periodontitis.Methods1.From November 2017 to November 2018,121 patients with cognitive dysfunction and simple moderate-to-severe periodontitis in the Department of Neurology and Stomatology of Liaoning Provincial People's Hospital were enrolled in the general information and oral hygiene habits questionnaire,and then given the standard Mini-mental state examination(MMSE)and periodontal clinical examination.They were divided into cognitive dysfunction with moderate-to-severe periodontitis group(experimental group),simple moderate-to-severe periodontitis group(control group).All subjects were between 65 and 75 years old and agreed to participate in the experiment and fill out the informed consent form.2.(1)General data was collected in patients with newly diagnosed,detailed record of the patient's age,gender,smoking history,educational level and brushing behaviors etc.(2)All subjects received the examination of the neurological scale,which was conducted by the investigator who had received cognitive training in neurology department and who filled in the contents of the scale.(3)All subjects underwent examination of all remaining teeth in the mouth.Each tooth recorded the six loci(proximal,central,and distal of the 1ip(buccal)and tongue).Inspection indicators: Probing depth(PD),Attachment loss(AL),Missing teeth(MT).(4)The plaque samples were collected by 4-site method,and the samples were fozen and stored at-70.(5)The 16 s r RNA fragments of P.g and T.d were amplified by polymerase chain reaction(PCR)method.The amplified products were loaded on 3% agarose gel and observed under UV observation.3.The spss 21.0 software package was used to analyze the data.Result1.Comparison of general data: patients with moderate-to-severe periodontitis(control group)and patients with moderate-to-severe periodontitis with cognitive dysfunction(experimental group)had no significant differences on age,gender,smoking,education level and other general data.(P>0.05).2.Comparison of oral hygiene habits:There were statistically significant differences in brushing habits and time between the two groups(P < 0.05),but no statistically significant differences in brushing methods(P > 0.05).3.When comparing the clinical indicators of severe periodontitis,PD? AL and MT in the experimental group were significantly different from those in the control group(P <0.05).4.Comparison of the results of the neurological scale:The MMSE score of the experimental group was 20.50±2.89 for severe periodontitis and 18.47±3.73 for moderate periodontitis,the difference was statistically significant(P < 0.05).5.Correlation between periodontal clinical indicators and neurological scale:There was a significant correlation between PD,AL,MT and MMSE score(P < 0.05),and the correlation coefficients were-0.207,-0.188 and-0.193,respectively,indicating that PD,AL,MT and MMSE had a weak negative correlation.6.Comparison of detection rates of the two types of periodontal pathogens : under different inflammatory conditions,the detection rates of P.g and T.d in the experimental group and the control group showed no significant difference(P > 0.05).Conclusion1.Patients with cognitive dysfunction and periodontitis have poor oral hygiene habits.2.The degree of periodontal tissue lesions in patients with cognitive dysfunction and periodontitis is more serious than that in patients with moderate to severe periodontitis.3.There may be an interaction between cognitive dysfunction and moderate to severe periodontitis. |
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