HLA-Cw*0602,HLA-DQB1*0602 Allele Detection Of Psoriasis Vulgaris In The Han Nationality In Lanzhou

Background:Psoriasis is a common chronic recurrent inflammatory skin disease whose etiology and pathogenesis are still unclear.Psoriasis is currently considered to be a systemic disease caused by a combination of genetic factors and environmental factors.Genetic factors have been identified as important locations in the pathogenesis of psoriasis through epidemiological data,HLA analysis and GWAS studies from multiple countries,regions,and ethnic groups.To date,86 gene regions have been found to be associated with psoriasis,with HLA being most closely related to psoriasis.There are few studies on the susceptibility genes of psoriasis in Lanzhou.The HLA-Cw*0602 and HLA-DQB1*0602 alleles of psoriasos vulgaris in Lanzhou area and their correlation with clinical phenotype have not been retrieved.Objective:To study the association between psoriasis vulgaris and HLA-Cw*0602,HLA-DQB1*0602 alleles in Han nationality in Lanzhou,and analyze the HLA-Cw*0602,HLA-DQB1*0602 allele at the age of onset(early-onset/late-onset),gender(male/female),type of onset(guttate/plaque),family history(positive/negative),and severity of skin lesions(light/medium/heavy).Method:Using high-resolution polymerase chain reaction with sequence-specific primers(PCR-SSP),the frequencies distribution of HLA-Cw*0602 allele and HLA-DQB1*0602 allele among 50 psoriasis vulgaris patients and 50 controls in Han nationality in Lanzhou area were detected and questionnaire survey Its clinical phenotypes.The database was built with SPSS22.0software and analyze the frequencies of allele.Results:1.This study included 50 patients with Han Chinese vulgaris psoriasis in Lanzhou,including37 males(74%)and 13 females(26%)with a mean age of(41.96±23.44)years.There were 50patients in the control group,including 24 males(48%)and 26 females(52%)with an mean age of(42.20±22.21)years.2.The results of HLA-Cw*0602 and HLA-DQB1*0602 alleles in the psoriasis vulgaris patients group and the control group:50 patients with psoriasis vulgaris were enrolled,50 patients with HLA-Cw*0602 allele,100%gene frequency;50 patients in the control group,50 patients with HLA-Cw*0602 allele,gene frequency 100%.In the PV group,36 patients with HLA-DQB1*0602 allele,with a gene frequency of 72%.In the control group,25 patients with HLA-DQB1*0602 allele,with a gene frequency of 50%.The HLA-Cw*0602 allele frequency did not differ between the PV group and the control group.The frequency of HLA-DQB1*0602 allele in the PV group was significantly higher than that in the control group,and the difference was statistically significant,?~2=5.09,P=0.02<0.05.3.The HLA-DQB1*0602 allele was detected in patients with early-onset and late-onset psoriasis vulgaris:The detection rate of HLA-DQB1*0602 allele in patients with early-onset psoriasis was 75%,and the detection rate of patients with late-onset psoriasis was 60%.The difference was not statistically significant,?~2=0.30,P=0.58>0.05.4.Detection of HLA-DQB1*0602 allele in patients with positive family history and negative psoriasis vulgaris:The detection rate of HLA-DQB1*0602 allele in family history-positive psoriasis vulgaris was 62.5%,and the detection rate in family history-negative psoriasis vulgaris was 73.81%.There was no significant difference between the two groups,?~2=0.50,P=0.82>0.05.5.The HLA-DQB1*0602 allele was detected in patients with guttate and plaque psoriasis vulgaris:The detection rate of HLA-DQB1*0602 allele in patients with guttate psoriasis was 50%,and the detection rate in patients with plaque psoriasis was 78.95%.There was no significant difference between the two groups,?~2=2.49,P=0.12>0.05.6.The HLA-DQB1*0602 allele was tested for psoriasis vulgaris in different genders:The detection rate of HLA-DQB1*0602 allele in male patients with psoriasis vulgaris was 70.27%,and the detection rate in female psoriasis vulgaris was 76.92%.There was no significant difference between the two groups,?~2=0.01,P=0.92>0.05.7.The HLA-DQB1*0602 allele was tested for psoriasis vulgaris in different severity:The detection rate of HLA-DQB1*0602 allele in patients with mild psoriasis was 62.96%,and the detection rate in patients with moderate psoriasis vulgaris was 64.29%.It was detected in patients with severe psoriasis vulgaris.The rate was 55.56%.There was no significant difference between the three groups,?~2=0.20,P=0.905>0.05.Conclusion:In this study,we performed HLA-Cw*0602 and HLA-DQB1*0602 genes in association with psoriasis vulgaris in Han nationality in Lanzhou,and further analyzed their association with the clinical phenotype of psoriasis vulgaris.The results showed that the HLA-Cw*0602 gene was positive in both the psoriasis vulgaris group and the control group,and the negative control strips were not abnormal,so the factors of specimen contamination could be excluded.The choice of primers may be the reason why the HLA-Cw*0602 gene is positive in both the experimental group and the control group.The HLA-DQB1*0602 gene may be a susceptible gene for psoriasis vulgaris in Han nationality in Lanzhou,but it is not associated with age at onset,gender,family history,type of lesion,and severity.