The Effects Of Modified Buwang Powder On The Expression Of NLRP3 Inflammasome And Related Inflammatory Cytokines

Objective:To investigate the effects of Modified Buwang Powder(MBWP)on the learning and memory ability of Alzheimer's disease(AD)model rats and the expression of NLRP3,Caspase-1and IL-1? in NLRP3 inflammatory pathway in hippocampus of Alzheimer's disease(AD)mouse model,and exploring the underlying mechanism of MBWP.Methods:1.The 52 eligible rats were screened through Morris water maze and randomly divided into sham control group,AD model group,low-dose MBWP and high-dose MBWP group.2.AD mouse model was established by bilateral hippocampus injection of A?1-42.Two week later,the mouse in low-dose and high-dose MBWP group received low(0.15g/ml,10ml/kg)and high dose(0.30g/ml,10ml/kg)MBWP respectively,while the mouse in AD model and sham control group received the same volume of physiological saline,and then all groups administrated every day between 8 am and 10 am by ig in for4 week.3.After the administration,Morris water maze was used to determine whether the AD model is successfully established and test the effect of MBWP on learning and memory ability.4.The expression of NLRP3,Caspase-1 and IL-1? mRNA was tested by RT-PCR.5.The expression of NLRP3,Caspase-1 and IL-1? proteins was tested by Westernblotting.Results:1.The results of Morris water maze showed that as compared with the sham group,the escape latency of the AD model group was significantly longer,and the rat's activity track was marginalized and randomized,suggesting that the AD mouse model was successfully established.When compared with AD model group,the escape latency in low-dose MBWP group was no statistical differences from the first to the fifth day,while in high-dose MBWP group was significantly shoter.In the space exploration test,as compared with the AD model group,the platform quadrant residence time and cross-platform times of the low-dose MBWP group was no statistical difference,while the platform quadrant residence time of the high-dose MBWP group was significantly prolonged,and cross-platform times was significantly increased.There was no significant difference in swimming speed between the groups,suggesting that has the interference of swimming speed on results could be basically eliminated.2.The results of RT-PCR showed that the expression of NLRP3,Caspase-1 and IL-1? mRNA in hippocampus of rats was significantly increased in AD model group compared with sham control group,which was no statistical differences in low-dose MBWP group,but significantly decreased in high-dose group MBWP compared with AD model group.3.The results of Western blot showed that the expression of NLRP3,Caspase-1 and IL-1? proteins in hippocampus of rats was significantly increased in AD model group compared with sham control group,which was no statistical differences in low-dose MBWP group,but significantly decreased in high-dose MBWP group compared with AD model group.Conclusion:1.AD model rats were successfully established by injecting A?1-42 into bilateralhippocampus.2.High-dose MBWP could be used to ameliorate the learning and memory ability of AD rats.3.The inflammatory level increased in the hippocampal tissue of AD model rats,which participate in AD inflammatory response.4.High-dose MBWP could attenuate neuroinfammation in the hippocampus via inhibiting the expression of NLRP3,Caspase-1 and IL-1?.