The Effects Of Modified-buwang-powder On The Changes Of Learning And Pathology In The APP/PS1 Mice Hippocampus

Objective:The aim of this study was to clarify the possible mechanism of the improvement of cognitive dysfunction in APP/PS1 transgenic mouse model by Modified-buwang-powder(MBWP)and to observe the pathological changes of hippocampus and neuronal apoptosis in the model mice.Methods:10 B6C3 mice(20g±2g)were randomly selected as B6C3 non-transgenic group(C group),which are 2 month old.And 30 APP/PS1 mice(20g ± 2g)which are 2month old were randomly divided into 3 groups(10 in each group),labeled as APP/PS1 transgenic group(referred to as group A),low-dose MBWP group(referred to as group L),and high-dose MBWP group(referred to as group H).Forty mice of the C,A,L,and H groups were kept at a constant temperature,2 per cage.Four groups of mice were administered from 4 months to 10 months of age,and the H and L groups were given high(0.3g/10g)dose drug and low(0.15g/10g)dose drug.B6C3non-transgenic group and APP /PS1 transgenic group were administered with normal saline(1 ml/10 g).At 9 months of age,the all mice were placed in the Morris water environment for adaptation.The mice were evaluated for learning and memory function at 10 months using the Morris test.After the Morris experiment,all the mice were decapitated,and the hippocampus were isolated and sectioned.The pathological changes of the hippocampus cells were observed by HE staining.The correlation between apoptosis and AD was observed by TUNEL staining.Results:1.The effect of MBWP on learning and memory in APP/PS1 transgenic mice:Compared with group C,there were significant differences in the indicators of group A,group L and group H(the escape latency,space exploration ability)(p<0.05).Compared with group A,the escape latencies of group L and group H of the group was significantly shortened on the 2nd to 5th day.Compared with the L group,the escape latency of the H group was significantly shortened on the2nd-5th day.2.The effect of MBWP on the spatial exploration ability of APP/PS1 transgenic mice:There was a difference in the residence time above the original quadrant about the four groups : the APP/PS1 transgenic group showed a significant decrease in the residence time compared with B6C3 non-transgenic group,and there was a statistical difference.(p<0.05)The low-dose MBWP group had a longer residence time in the original platform quadrant than in the APP/PS1 transgenic group;the high-dose MBWP group had a lower dose of MBWP on the original platform.The quadrant stay time has been extended.For the mean times of passing platform,the APP/PS1 transgenic group was significantly reduced compared with the B6C3 non-transgenic group.There was a difference between the APP/PS1 transgenic group and the low-dose MBWP group and the high-dose MBWP group.(p<0.05)At the same time,we can see that the high-dose MBWP group had a slightly more times than the high-dose MBWP group.3.HE staining results: The hippocampus neurons in the APP/PS1 transgenic group and the low-dose MBWP group were significantly reduced,and the cell volume was reduced,and the cell nucleus turned less with karyopyknosis.the blank control group and high dose The number of hippocampal neurons in the group was significantly increased.The cells were arranged tightly and neatly,and the nuclear morphology was consistent with the age of the mice.4.TUNEL staining results: the apoptotic rate of neurons in the hippocampus of the B6C3 non-transgenic group,APP/PS1 transgenic group,low-dose MBWP group,and high-dose MBWP group: 5.35±0.88,40.44± 5.17,33.39 ± 5.19,22.60 ± 4.60.A large number of apoptotic neurons were observed in the hippocampus of APP/PS1 transgenic mice,whch was more significantly increased compared with the B6C3 non-transgenic group.(p<0.05)The number of apoptotic cells in the low-dose MBWP group was significantly different from the APP/PS1 transgenic group.(p<0.05)The number of apoptotic cells in the high-dose MBWP group was significantly reduced compared with the APP/PS1 transgenic group,which was lower than the low-dose MBWP group.(p<0.05)conclusion:1.MBWP can improve the learning and memory function of APP/PS1 transgenic mice.The effect of low-dose MBWP is not significant.The high-dose MBWP has improved learning and memory ability in mice.2 Alzheimer's disease can cause pathological damage in the hippocampus of APP/PS1 transgenic mice,and the use of MBWP can alleviate it.3.MBWP can significantly improve the apoptosis of hippocampus neurons in the brain of APP/PS1 transgenic mice,which may be the main mechanism of MBWP treatment of AD,and it is concentration dependent.