The Expression And Clinical Significance Of S100A9 And MMP-9 Protein In Pancreatic Carcinoma

Objective:To detect the expression of Ca-binding protein S100A9 and matrix metalloproteinase-9 protein in pancreatic cancer tissues and explore its clinical significance.Methods:The histopathologic slides and clinical data of 56 patients with pancreatic cancer,who underwent surgical resection in the First Affiliated Hospital of Nanchang University from January 2013 to December 2017,were collected.Streptavidin-perosidase(SP)immunohistochemical method was used to detect the expression of S100A9 and MMP-9 proteins in pancreatic cancer tissues and corresponding paracancerous tissues.The relationship between their expression and its correlation with clinicopathological parameters of pancreatic cancer were analyzed.Results:1.S100A9 protein was expressed in both cytoplasm and nucleus.The expression of S100A9 in pancreatic carcinoma tissues and paracancerous tissues were 69.64%(39/56)and 35.71%(20/56),respectively.The expression of S100A9 in pancreatic carcinoma tissues was significantly higher than that in paracancerous tissues(P=0.000).The expression of S100A9 in pancreatic carcinoma was significantly correlated to histological grades(P=0.001),nervous invasion(P=0.001),lymphatic metastasis(P=0.000)and TNM staging(P=0.018),but was not correlated to age(P=0.867),sexuality(P=0.487),and T stage(P=0.629).2.MMP-9 protein was predominantly expressed in cytoplasm.The expression of MMP-9 in pancreatic carcinoma tissues and paracancerous tissues were 71.43%(40/56)and 32.14%(18/56),respectively.The expression of MMP-9 in pancreatic carcinoma tissues was significantly higher than that in paracancerous tissues(P=0.000).The expression of MMP-9 in pancreatic carcinoma was significantly correlated to nervous invasion(P=0.005),lymphatic metastasis(P=0.002)and TNM staging(P=0.014),but was not correlated to age(P=0.608),sexuality(P=0.866),T stage(P=0.521)and histological grades(P=0.252).3.The Spearman correlation analysis showed a positive correlation between the expression of S100A9 and MMP-9 protein in pancreatic carcinoma(r=0.614,P=0.000).Conclusions:1.S100A9 and MMP-9 proteins are aberrantly up-regulated in pancreatic cancer,which are closely associated with the progress of pancreatic cancer.2.The expression of S100A9 and MMP-9 is positively correlated in pancreatic cancer.3.Combined detection of S100A9 and MMP-9 protein expression may be helpful to the diagnosis and prognosis of pancreatic cancer.