The Expression And Clinical Significance Of Golgi Phosphorpro-tein 2、MCM2 And Ki-67 In The Tissue Of Pancreatic Cancer

The malignant of pancreatic cancer is high,and the prognosis of pancreatic is poor. At present there is no effective tumor marker,and we also can’t do early diagnosis and early treatment, so the pancreatic cancer is often in a terminal cancer. At this time,the resection rate is low, the prognosis is poor,and mortality is almost close to its incidence Now the only effective way to treat pancreatic cancer is surgical resection. However, early symptoms of pancreatic cancer is lighter, Even there is no clear clinical symptoms, so to achieve early diagnosis of pancreatic cancer is very difficult, Now pancreatic cancer patients that are able to be early diagnosis and effective surgical treatment is less than 20% of the incidence of pancreatic cancer.Tumor metastasis and recurrence of pancreatic cancer reduced the survival time of patients, causing the failure of the surgical treatment of pancreatic cancer. In order to alleviate clinical symptoms of patients with the terminal cancer, In order to effectively prolong patient’s postoperative survival and to improve the quality of life,the medical workers have done a lot of effort and trying,and the chemotherapy is one of them, although there are some help for the treatment of cancer, the effect is not ideal. To find the positive and effective tumor markers, becomes very important in the present moment.Golgi phosphoprotein 2(GOLPH2) is a new type of epithelial cells specificity transmembrane protein, its relative Ⅱmolecular mass is 73000, it is also called GP73. After in-depth study we found that GOLPH2 can be detected in a variety of normal tissues, and GOLPH2 over-expressed in a wide variety of tumor cells, So GOLPH2 could be a important carcinoma protein. It has to do with closely related to the occurrence, development and metastasis of tumor, and there is great significance of diagnosis and treatment of tumor.Small chromosomes maintain protein 2(MCM2) is one of the most important role in six subunits of the micro chromosomes maintain protein family MCMs. It exists in the nucleus of the cell cycle. In the normal cells, in different cell cycle the m RNA level of MCMs protein was not exactly the same,during G1 phase and S phase of the expression of MCMs protein achieve the maximum, such characteristics that accompanied by the cell cycle change and its expression level is changed, just can be used to determine cell proliferation in which period. Now MCM2 which has considered iconic protein of S phase cells is the specific proliferation related factors and precancerous markers, and for the early diagnosis of tumor and prognosis have important value.The cells hyperplastic antigen Ki – 67 is a kind of cell cycle-related proteins. Ki- 67 exists in each cell proliferation cycle except G0, Ki – 67 begin to express in G1 middle-late, increase gradually in S phase and G2 phase, reach the peak in M period. Confirmed by research, Ki- 67 expressed only in the proliferation of cells, It do not express in the cell stationary phase, At present it has been as an important symbol of cell proliferation, and is used to reflect the tumor cell proliferation activity and proliferation rate.The study by detecting the expression level of GOLPH2, MCM2 and Ki- 67 protein in pancreatic cancer, explore the correlation of GOLPH2 protein and MCM2 protein and GOLPH2 protein and Ki- 67 protein in pancreatic cancer,analysis the relations with pancreatic cancer clinical indicators among MCM2 、GOLPH2 and Ki – 67. Objective:By immunohistochemical method to detect the expression level of GOLPH2, MCM2 and Ki- 67 three proteins in pancreatic cancer tissue and in normal tissue,to analysis the relations with pancreatic cancer clinical indicators among MCM2 、GOLPH2 and Ki – 67,to explore the role of GOLPH2, MCM2 and Ki- 67 in occurrence and development of pancreatic cancer, to provide the reliable theory basis of clinical treatment and surgery prognosis judgement for pancreatic cancer patients. Methods:Collecting 56 cases pathological wax block of pancreatic cancer tissue which has been surgical resection and postoperative pathological diagnosis, 30 cases pathological wax block of normal pancreatic tissue adjacent to carcinoma, by using the immunohistochemical of Envision to detect the expression level of GOLPH2, MCM2 and ki- 67 protein in pancreatic cancer, and to analysis the relations with pancreatic cancer clinical pathological features and MCM2 GOLPH2 and Ki–67. The data of Immunohistoche-mistry were analyzed by using SPSS 17.0 for windows,using the c2 test and Fisher exact test more positive to analyze the expression of MCM2 、GOLPH2 and Ki – 67 and to analyze the relationship of MCM2 、GOLPH2 and Ki – 67 with the clinicopathologic features of pancreatic cancer;using the Spearman correlation analysis to analyze the correlation between MCM2 、GOLPH2 and Ki – 67,α = 0.05 as inspection standard, P < 0.05 for differences with a statistical significance. Results:1. The expression of GOLPH2 positive rate was 73.20%(41/56) in pancreatic cancer tissue, while it was 23.3%(7/30) in normal pancreatic tissue. The positive expression rate is significantly higher in pancreatic cancer tissue for GOLPH2 than that is in normal pancreatic tissue, the difference was statistically significant(P < 0.05).2. MCM2 protein expression in pancreatic cancer tissue of the positive rate was 67.86%(38/56), its expression in normal pancreatic tissue positive rate was 10.00%(3/30). The positive expression rate in pancreatic cancer tissue for MCM2 is significantly higher than that is in normal pancreatic tissue adjacent to carcinoma, the difference was statistically significant(P < 0.05).3. The ki- 67 protein expression in pancreatic cancer tissue of the positive rate was 71.43%(40/56), its expression in normal pancreatic tissue positive rate was 30.00%(9/30). The positive expression rate in pancreatic cancer tissue for Ki-67 is significantly higher than that is in normal pancreatic tissue adjacent to carcinoma, the difference was statistically significant(P < 0.05).4. GOLPH2 protein expression in pancreatic cancer tissue and tumor stage, tumor size and tumor differentiation degree, the lower the degree of tumor differentiation, the late tumor stage, tumor diameter, the greater the GOLPH2 had higher positive rate(P < 0.05), and has nothing to do with the patients age and sex(P > 0.05).5. MCM2 positive expression was 90.24% that is in positive group of GOLPH2, higher than the 26.67%, expressed in the negative group in the between statistically difference(P < 0.05). GOLPH2 positive expression and MCM2 positive expression was positively correlated(P < 0.05). GOLPH2 positive in the positive expression of ki- 67 with 90.24%, 20.00% higher than the expression of ki- 67 in the negative group, statistical differences between the two(P < 0.05). GOLPH2 positive expression and Ki- 67 positive expression was positively correlated(P < 0.05).Conclusions:GOLPH2, MCM2 and Ki-67 in pancreatic cancer tissues was expressed, the positive expression rate was significantly higher than that of normal tissue adjacent to carcinoma, and GOLPH2 and MCM2 GOLPH2 and Ki-67 expression levels were positively correlated. GOLPH2 expression level and has no impact on patients’ gender, age, related to the size and differentiation degree and clinical staging of pancreatic cancer, along with the degree of differentiation of pancreatic cancer reduced GOLPH2 expression level increased, and GOLPH2 in clinical stage for Ⅲ- Ⅳexpression level was significantly higher in the group in the clinical stage of Ⅰ- Ⅱgroup expression level, prompt GOLPH2 excessive expression is closely related to the occurrence and development of pancreatic cancer, it is a sensitive index in the process of tumor development. Three collaborative process involved in the occurrence and development of pancreatic cancer, the joint detection GOLPH2, MCM2 and Ki- 67 in judging metastasis potential and prognosis of pancreatic cancer has a guiding significance.