Tlr9 Expression In Pancreatic Cancer And Its Clinic Significance

Objective: To investigate the expression of Toll-like receptor 9 (TLR9) in pancreatic cancer and to explore their clinical significance. Methods: The real-time PCR technique, western blot method and immunohistochemical method were used to examine the expression of TLR9 in the pancreatic cancer, tissues near tumor, and normal pancreatic tissues which obtained during operation from 30 pancreatic carcinoma patients and 10 normal non-lump patient. The relationships with pathological grade, clinical stage, and metastasis of pancreatic cancer were statistically analyzed.Results:1. The amplification value level of TLR9mRNA expression in human pancreatic tissues, paracancerous tissues were 2.32(1.41～3.22)and 1.23(1.18～1.28),respectively(t=2.642,p=0.023).2. The percentage of positive cells expressed TLR9 protein in human pancreatic tissues, paracancerous tissues and normal tissues were 73.3%, 33.3% and 20.0%,the protein expression level of TLR9 was gradually descending (χ2=13.99,P=0.001).3. The highly expressed TLR9 correlated significantly with the degree of tumor differentiation,an advanced TNM stage and lymph node metastasis. Similar result was obtained by Western blot. Conclusions:1. TLR9 has a higher expression in pancreatic cancer tissues. The change of the proteins may play an important role in development of pancreatic cancer.2. TLR9 expression has a close relationship with pathological grades of pancreatic cancer, suggesting that TLR9 play an important role in the development and progression of pancreatic cancer through immunologic mechanism.3. The first demonstration of TLR9 in pancreatic cancer and providing a histological basis for further explore the signal conditioning in pancreatic cancer.