| Objective : 1.On the basis of modern literature,this paper discusses the common clinical diseases and TCM syndromes involved in the symptoms of "tidal fever",and provides reference basis for clinical diagnosis and treatment of diseases and differentiation of symptoms and signs.2.In this experiment,an animal model of postmenopausal syndrome was established by ovariectomy.They were randomly divided into 5 groups: blank control group,disease model group,disease treatment group and syndrome treatment group.To observe the changes of body temperature,body weight,sex hormone,hypothalamus 5-HT and uterus,to explore the biological mechanism of kidney deficiency syndrome based on hypothalamus-pituitary-gonadal axis menopause syndrome.Methods:1.From 2008 to 2017,the related literatures related to "tidal fever" were searched,the contents of disease and syndrome were extracted from the literature,and the syndromes were divided into syndrome elements for statistical analysis.2.n female SD rats,50 rats were randomly divided into 5 groups(10 in each group):the blank control group,the disease model group,the disease treatment group,the disease-proof model group and the disease-proof model treatment group.The treatment group of the disease model group,the disease treatment group,the disease-proof model group and the disease-proof model group were treated with bilateral oophorectomy,and the same operation was performed in the blank control operation group,but the ovaries were not removed.In the disease treatment group,after ovariectomy,the estradiol valerate solution was started and 20 ml/ kg continued for 4 weeks.In the treatment group,the rats were treated with Zuogui Pill on a daily basis,and the treatment was continued for 4 weeks.Blank control group,disease model group and disease certificate Combined with the model group,the same volume distilled water was given at the same time for 4 weeks.The tail temperature of each group was measured 1 hour after daily treatment.Infra-red thermometer was used to measure the tail of the rats and the mean values were calculated three times.The uterus of rats was extracted,the weight of uterus was measured accurately,andthe index of uterus was calculated.Serum E2(estradiol),FSH(follicle stimulating hormone),LH(luteinizing hormone),GnRH(gonadotropin releasing hormone)and5-HT(pentahydroxytryptamine)were measured by enzyme linked immunosorbent assay(Elisa).The differences of observed indexes in each group were compared and analyzed.Results:1.Literature study: a total of 470 articles were included,in which 75 kinds of symptoms of "tidal fever" were involved in the literature,38 syndromes,7syndrome elements and 14 syndrome elements were involved in the literature.2.Experimental study results(1)The change of weight of rats: Compared with the blank control group,the weight of the rats in the disease model group and the disease-proof combined model group was significantly increased(p <0.01).After 4 weeks of drug treatment,the trend of weight gain in the disease treatment group decreased(p <0.01)as compared to the disease model group.The trend of body weight growth in the treatment group was decreased(p <0.01)compared with the model group.(2)the change of tail temperature in rats: compared with the blank group,the tail temperature in ovariectomized rats was higher than that in the disease model group,and the mean body temperature in the disease treatment group was significantly lower than that in the disease model group(P < 0.05).Compared with the disease treatment group,there was no significant difference in the mean body temperature between the disease treatment group and the disease treatment group(P > 0.05).(3)uterine index: compared with the blank control group,the uterine weight and coefficient of uterus in each group decreased significantly(P < 0.01).Compared with the disease model group,the weight of the frontal uterus and the uterine coefficient in the treatment group did not increase significantly(P > 0.05).Compared with the model group,the weight of uterus and the coefficient of uterus did not increase significantly in the model group(P > 0.05).(4)Rat uterus General morphological observation of uterine tissue: blank control group,the appearance of red color,moderate thickness;model group slight atrophy,light red appearance color,some tissues showed signs of mild edema;treatment groupappearance of dark red,hyperemia.Under optical microscope: blank control group: normal shape,clear structure,normal endometrium,adventitia,myometrium and endomembrane,abundant glands and capillaries,large glandular cavity and loose connective tissue in rats;In the disease model group,the size of the uterus became smaller,the wall of the uterus thinner,the lumen of the uterus smaller,the number of uterine glands decreased,the glands narrowed,the myocytes and glandular cells atrophied,and because of the shrinking volume,the cells were densely arranged.The intima became thinner and the glands and capillaries were few.After drug intervention,the endometrium was thickened and the glands and capillaries were obviously increased in each treatment group.In the treatment group,the endometrium showed excessive thickening,endometrial cells were edema,and the arrangement of endometrial cells was looser than that of the control group(P < 0.05).(5)the changes of serum E2 in rats: compared with the blank control group,the levels of E2 in the disease model group and the disease model group decreased significantly(p < 0.01),and the E2 level in the disease treatment group increased significantly compared with the disease model group(p < 0.01).Compared with the model group,the E2 level in the treatment group was significantly higher than that in the model group(p < 0.01),but there was no significant difference between the treatment group and the treatment group(p > 0.05).(6)the changes of serum FSH in rats: compared with the blank control group,the serum FSH level in the disease model group and the disease model group increased significantly(p < 0.01),and the serum FSH in the disease treatment group decreased significantly compared with the disease model group(p < 0.01).Compared with the model group,the serum FSH in the treatment group was significantly decreased(p < 0 01),but there was no significant difference between the treatment group and the disease treatment group(p > 0 05).(7)The changes of serum LH in rats: compared with the blank control group,the LH level of the disease model group and the disease-proof model group was significantly increased(p <0.01);there was no significant difference in serum LH between the disease model group and the disease treatment group(p> 0.05);compared with thedisease-proof model group,There was no significant difference in serum LH in the treatment group(p> 0.05),and there was no significant change in serum LH after treatment.(8)the changes of serum GnRH in rats: compared with the blank control group,the level of GnRH in the disease model group and the disease model group was significantly higher than that in the disease model group(p < 0.01),and the GnRH in the disease treatment group was significantly lower than that in the disease model group(p < 0.01).Compared with the model group,the GnRH in the treatment group was significantly lower than that in the model group(p < 0 01),but there was no significant difference between the disease treatment group and the disease syndrome treatment group(p > 0 05).(9)the changes of 5-HT in rats: compared with the blank control group,the level of5-HT in the disease model group and the disease model group was significantly lower than that in the disease model group,and the 5-HT level in the disease treatment group was significantly higher than that in the disease model group(p < 0.01).Compared with the model group,the level of 5-HT in the treatment group was significantly higher than that in the model group(p < 0.01),but there was no significant difference between the disease treatment group and the disease-syndrome treatment group(p > 0.05).Conclusion:1.As a common clinical symptom,"tidal fever" involves a variety of diseases and various syndromes.It is of great clinical practical value to distinguish diseases from the main symptoms of "tidal fever" and to diagnose and treat them according to differentiation of symptoms and signs.2.After treatment with Zuogui pills and estrogen,it was found that Zuogui pills could regulate E2,FSH,LH levels in menopause model rats,strengthen the negative feedback to FSH and LH,and adjust the dysfunction of hypothalamus-pituitary-ovary axis.Zuogui pills may inhibit the synthesis of 5-HT in hypothalamus of ovariectomized rats to treat autonomic nerve dysfunction.The therapeutic effect of Zuogui pills on kidney deficiency syndrome in rats with menopause syndrome is good,which indicates that kidney deficiency is closelyrelated to hypothalamus-pituitary-gonadal axis. |
PDF Full Text Request