| BackgroundPreeclampsia(PE)is a disease unique to women during pregnancy.It is mainly characterized by hypertension,edema,proteinuria and other symptoms after 20 weeks of gestation,causing systemic dysfunction and even irreversible damage in pregnant women.The incidence rate is around 5%,which seriously threatens maternal and child health.Therefore,timely and effective treatment is of great significance for the prognosis of PE patients.At present,the pathogenesis of PE is still unclear,and the theory of placental shallow implantation and vascular endothelial injury is the main theory.Tumor necrosis factor-?(TNF-?)acts as a core factor of inflammatory response.Excessive production can directly damage vascular endothelial cells,causing re-casting of placental spiral arterioles,causing placental shallow implantation,leading to placental ischemia and hypoxia.Nitrogen Monoxide(NO)is the most important component of many endogenous vasodilators released by endothelial cells.At present,the consensus on the cause of PE is the damage of vascular endothelium in vivo,NO synthesis,release and activity.Inhibition and destruction further lead to abnormal contraction of blood vessels and functional dysfunction,which further aggravates local or systemic ischemia and hypoxia,and finally forms a vicious circle,which makes the disease appear to deteriorate.TMP injection has blood circulation,dilatation of blood vessels,anti-platelet aggregation,elimination of vascular free radicals,protection of vascular endothelial cells,and improvement of cell tolerance to hypoxia.Studies have shown that the use of ligustrazine(TMP)injection in PE has a certain clinical effect,but whether it has affected the changes of clinical parameters such as blood pressure by reducing TNF-?and increasing NO level has not been reported.This clinical trial study used 60 patients with PE admitted to the Xinxiang City Maternal and Child Health Hospital as the research object,through the effects of different treatment regimens on the levels of TNF-?and NO in PE patients before and after treatment,to explore the significance of TMP injection on the clinical efficacy of PE.ObjectiveTo investigate the effect of TMP injection on the expression TNF-?and NO in PE patients,the purpose of this study was to investigate the mechanism of TMP injection on the clinical efficacy of PE.Method1 research objecta total of 60 patients who were randomly diagnosed,hospitalized and diagnosed with PE in Xinxiang City Maternal and Child Health Hospital from January 2018 to September 2018,The patients and their families who were included in the experiment were informed of the contents of the study and signed informed consent,and were reviewed and approved by the Ethics Committee of Xinxiang City Maternal and Child Health Hospital.2 experimental methodsBoth the experimental group and the control group were treated routinely.In addition to conventional treatment,the control group was given an intravenous infusion of 25% magnesium sulfate.In the experimental group,on the basis of conventional treatment plus intravenous infusion of magnesium sulfate.Take 10 days as a course of treatment.All patients included in the experiment were in the early morning after 12 hours of fasting,taking 6 ml of elbow vein blood in a quiet state,gently invert and mix,all venous blood samples were allowed to stand at normal room temperature for1-2 h,then centrifuged at 1000 r per minute for 20 minutes,and the supernatant was stored in a-80? refrigerator for centralized detection to avoid repeated freezing and thawing.The content ofTNF-? in serum was determined by enzyme linked immunosorbent assay(ELISA),and the level of NO in serum was determined bynitrate reduction method.Result1 The results showed that there was no significant difference in the expression level of TNF-? between the experimental group and the control group.The results were: control group(18.15 ± 7.23)ng/ml,experimental group(18.23 ± 6.78)ng/ml.After treatment,they were: control group(9.81 ± 4.72)ng/ml,experimental group(7.18 ± 3.56)ng/ml.In the control group,the level of TNF-? decreased significantly after treatment compared with the treatment group.TheTNF-? level in the experimental group was more obvious after treatment than before treatment.After treatment,the levels of TNF-? in the experimental group and the control group were compared between the two groups.The difference between the two groups was statistically significant(P<0.05).2 There was no significant difference in NO expression levels between the experimental group and the control group before treatment: control group(3.46±1.61)? mol/L,experimental group(3.48 ± 1.52)? mol /L.The expression levels after treatment were: control group(4.08 ±0.89)?mol/L,experimental group(4.66±0.99)?mol/L.The expression of NO in the control group was significantly higher than that before treatment.The expression level of NO in the experimental group was significantly higher than that before treatment.After treatment,the expression levels of NO in the experimental group and the control group were compared between the two groups.There was significant difference between the two groups(P<0.05).3 There was no significant difference in blood pressure between the experimental group and the control group before treatment.The experimental group was 173.63±7.8/116.50±8.87 mmHg,and the control group was 172.72±8.82/115.61 ± 9.49 mmHg.Blood pressure levels were measured again after treatment.The results were:experimental group 145.22±4.32/98.55±3.78 mmHg,and control group 150.31±9.27/103.26±7.86 mmHg.In the control group,the blood pressure level decreased significantly after treatment compared with the treatment group.The blood pressure of the experimental group was more obvious after treatment than beforetreatment.After treatment,the blood pressure levels of the experimental group and the control group were compared between the groups,and there were significant differences(P<0.05).The effective number of cases in the experimental group was 28,the overall effective rate was 93.0%,and the effective number in the control group was 22,and the overall effective rate was 73.0%.In contrast,the overall clinical efficiency of the experimental group was higher,There was significant difference(P<0.05)In conclusionLigustrazine injection has obvious therapeutic effect on patients with preeclampsia by reducing TNF-? and increasing NO level. |
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