Experimental Study On The Effect Of Tourniquet Application On Rabbit Bone-bone Cement Interface

Objective: To compare the biomechanical stability and microstructure of rabbit bone –bone cement interface formed by bone cement and cancellous bone with tourniquet and no tourniquet.The effect of tourniquet on the formation process of bone cement-bone interface was analyzed.Methods: 20 adult rabbits were randomly divided into four groups: mechanical experimental group(A1),mechanical control group(A2),microstructure experimental group(B1)and microstructure control group(B2).The mechanical experiment group(A1)and the microstructure experimental group(B1)were used to simulate the tourniquet application.The bone cement was bonded to the cancellous bone with tourniquet.In mechanical control group(A2)and microstructure control group(B2),the bone cement was bonded with cancellous bone without tourniquet.Then the tensile test was done on the mechanical experimental group and the mechanical control group.The maximum tensile load and the displacement were recorded the bone cement-bone interface fracture.The microstructure experimental group and the microstructure control group were made into bone cement-bone interface specimens.The bone cement-bone interface was observed by scanning electron microscope(SEM),and measured the maximum depth of the cement entering into the cancellous bone.The maximum tensile load and displacement were compared between the mechanical experimental group and the mechanical control group.The maximum depth of the cement entering into the cancellous bone was compared between the microstructure experimental group and the microstructure control group.Results: The average maximum tensile load of 5 specimens was 200.70 ±18.65 N and the displacement was 1.23 ±0.11 mm in A1 group.The maximum tensile load of 5 specimens was 187.08 ±27.54 N and the displacement was 1.13 ±0.19 mm in A2 group.There was no significant difference in maximum tensile load and displacement between the two groups(P > 0.05).The average maximum depth of the cement entering into the cancellous bone is 1.19±0.12 mm in B1 group;The maximum depth of the cement entering into the cancellous bone is 1.13±0.11 mm in B2 group,;There was no significant difference in the maximum depth between the two groups(P > 0.05).Conclusion: We use of tourniquet to reduce the bleeding of cancellous bone,it did not increase the initial stability of the bone cement-bone interface formed by bone cement and trabecular bone.And the penetration depth of bone cement in cancellous bone is not increased.