The Relationship Between Human Papillomavirus And Salivary Gland Tumor In Fujian

Background: High-risk human papillomavirus(HR-HPV)is significantly associated with head and neck squamous cell carcinomas(HNSCCs).Compared with non-HPVrelated ones,HPV-related head and neck squamous cell carcinoma(HNSCC)exhibiting unique clinical presentation and molecular biological characteristics,as well as favorable survival.Although salivary gland tumors play an important role in head and neck oncology,compared with squamous cell carcinoma,there are still very controversial result in the few published studies on the relationship between HPV and SGTs recently.Here,this clinically based study to assess the relationship between HPV and SGTs specifically in a cohort from in Fujian through multimethod.This research includes two parts as followed.1.The relationship between human papillomavirus and salivary gland tumor in 70 patients in Fujian Province.Objective: To investigate the relationship between HPV infection and the development of salivary gland tumors in in Fujian Province.Methods: This study included a total of 70 patients with primary SGTs from 2016 to 2017 in the Department of Oral and Maxillofacial Surgery of First Affiliated Hospital of Fujian Medical University.HPV infection in SGTs was detected by general GP5+/GP6+ polymerase chain reaction(PCR)combined with sequencing detected HPV infection in frozen tissue samples.HR-HPV16/18 status and significant biomarkers alterations were determined by HR-HPV16/18 E6 reverse PCR reaction(RT-PCR)and PIK3 CA hotspot mutation sequencing in this salivary gland tumor cohort.Results: 1.In all 70 SGTs cases between 2016 and 2017,only nine tumor tissues(12.9 %)were HPV positive detected by high sensitive general GP5+/GP6+ PCR,which was significantly higher than the proportion matched gland tissues(P=0.005)in parotid gland,of which HR-HPV18(55.5%)was the most common subtype.2.We found no significant correlations between HPV status and PIK3 CA mutations in this study.3.None was identified HPV16/18 E6 transcription by high-sensitivity RT-PCR in all 140 salivary gland tumors and adjacent normal fresh frozen tissue from 2016 to 2017.2.The relationship between human papillomavirus and salivary gland tumor in 133 patients in Fujian Province.Objective: To investigate the relationship between HPV infection and the development of salivary gland tumors in in Fujian Province.Methods: A total of 133 patients with primary SGTs from 2007 to 2017 in the Department of Oral and Maxillofacial Surgery of First Affiliated Hospital of Fujian Medical University.HPV infection in salivary gland cancer was detected by HR-HPV16/18 chromogenic in situ hybridization(CISH).HPV16/18 E6 and p16INK4 a immunohistochemistry(IHC)methods.Furthermore,we evaluate potential prognostic value of HR-HPV status and related significant molecular markers alterations in this salivary gland cancer cohort.Results: 1.In all 78 salivary gland cancer cases from 2007 to 2017,none was identified HR-HPV16/18 infection by CISH.2.HR-HPV16/18 E6 expression was not detected in all 133 patients by IHC methods.2.Dissimilar from HPV-related o head and neck squamous cell carcinoma(HNSCC)p16INK4a expression patterns,we found no significant correlations between HPV status and p16INK4 a expression in this study yet.3.Besides,though histopathology of malignancy,tumor size and regional lymph node metastasis of salivary gland carcinoma shown independent prognostic value in salivary gland carcinoma.Survival analysis cannot support the HR-HPV16/18 status and p16INK4 a expression independent prognosis value in 78 salivary gland cancer cases cohort.Conclusion: 1.Salivary glands are potential target for HPV infection in this study cohort,of which HR-HPV18 was the most common subtype.2.High-risk HPV was not implicated in the salivary gland tumors like HPV-related head and neck squamous cell carcinomas patterns in this study cohort from Fujian.