The Expression Of OPN In Serum And Saliva Of Patients With Recurrent Aphthous Ulcer

Objective: To explore the relationship between recurrent aphthous ulcer(RAU)and osteopontin(OPN).By measuring the content of OPN in the serum and saliva of RAU patients and the control group to explore the role of OPN in the pathogenesis of RAU.And by analyzing the correlation of OPN content in serum and saliva of RAU patients to further explore whether saliva can be the replacement of serum as a new detection method for the clinical detection of OPN and RAU.Methods: Choosing thirty patients randomly with MiRAU who were treated with periodontal mucosal disease at the Affiliated Stomatological Hospital of Jiamusi University were enrolled in this study.Among them,17 males and 13 females were selected to meet the diagnostic criteria of MiRAU history and clinical features.In addition,at the same period in the medical examination department of Jiamusi University Affiliated Stomatological Hospital 30 healthy people were selected randomly as the control group.Subjects were fasted in the early morning,and 5 ml of venous blood was collected with a dedicated serum separator.After standing,the supernatant was centrifuged to remove 0.5 ml of the supernatant and frozen in a refrigerator at-80°C.Saliva sample should be collected between 8 and 10 am.After rinsing with clean water,use a disposable needle to extract 2 ml of saliva,place it in a centrifuge tube,and keep the supernatant 0.5 ml at-80°C as well after centrifugation for 20 minutes.The sample was thawed at room temperature right before the experiment,and measuring the content of OPN in saliva and serum samples by enzyme-linked immunosorbent assay(ELISA).Results: Statistical analysis showed that: 1.The content of OPN in the saliva of the RAU group was(25.81±1.86)?g/L,and the content of OPN in the saliva of the control group was(22.63±1.51)?g/L.The RAU group's content of OPN was significantly higher than the control group's.The difference between the two groups was statistically significant(P<0.01).2.?g/L,and the content of OPN in serum of control group was(28.48±1.74)?g/L.Compared with the control group,the level of OPN in the serum of the experimental group was significantly increased,and the difference between the two groups was statistically significant(P<0.01).The correlation analysis showed that the content of OPN in serum and saliva of RAU patients was positively correlated and the correlation was statistically significant(r=0.551,P<0.05).Conclusions: 1.The expression of OPN in serum and saliva of RAU patients was significantly higher than that of normal control group.2.There was a positive correlation between the OPN content in saliva and serum of RAU patients.