Study On The Correlation Between Qi-Yin Deficiency,Phlegm(Wet) Heat Mutual Card And NO?SOD?MDA?GSH-Px In Type 2 Diabetes Mellitus

Objective:To investigate the expression of NO,SOD,GSH-Px and MDA in the serum of patients with type 2 diabetes mellitus with deficiency of both Qi-Yin and Phlegm(Wet)heat syndrome,and to investigate the expression of type 2 diabetes mellitus Antioxidant treatment for reference.Method:According to the subjects involved in the standard and the exclusion criteria,clinical cases were collected,divided into Qi-Yin Deficiency Syndrome group(group A)and Phlegm(Wet)heat mutual evidence group(group B),each group of 50 patients,at the same time Fifty healthy subjects of the same period were randomly selected as healthy control group(C group).The general data of patients and the related indexes of clinical examination were collected.Meanwhile,the levels of NO,SOD,GSH-Px and MDA in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the relative indexes of three groups of patients were compared.Results:1.There was significant difference in the course of disease between the two groups(P <0.05).There was no significant difference in age,sex and body mass index(P> 0.05).2.Three groups of indicators of differences:(1)compared with the C group,A and B group of FPG,2h PG,Hb A1 c,TC,TG,LDL-C and HDL-C differences were statistically significant(P> 0.05).(2)The levels of NO,SOD,MDA and GSH-Px in the three groups were statistically different(P <0.05).The levels of NO,SOD,MDA and GSH-Px in group A and group B were significantly different from those in group C(P <0.05)The levels of SOD and GSH-Px were significantly decreased,with significant difference(P <0.05).3.There was a positive correlation between MDA level and 2hPG level in group A(r =-0.305,P <0.05);TC level was significantly correlated with TG and LDL-C(R = 0.597,P <0.01),(r= 0.631,P <0.01).There was a negative correlation between TG level and HDL-C level(r =-0.325,(R = 0.729,P <0.01).There was no significant correlation between other indexes(P>0.05).(2)There was a significant positive correlation between NO level and SOD level in group B(r =-0.728,P <0.01).The level of TC was positively correlated with TG and LDL-C levels(r = 0.461,0.05),(r = 0.753,P <0.01).There was a significant positive correlation between FPG and 2h PG and Hb A1c(r = 0.646,P <0.01)Correlation(P> 0.05).Conclusion:1.The key factor of oxidative stress in the distribution of type 2 diabetes mellitus with deficiency of both Qi-Yin Deficiency Syndrome,Phlegm(Wet)heat mutual is that the levels of NO and MDA in Qi-Yin Deficiency syndrome are obviously higher than that in Phlegm(Wet)heat,The levels of SOD and GSH-Px were significantly lower than that of Phlegm(Wet)heat-interactive type.The patients with type 2 diabetes of Qi-Yin Deficiency syndrome had weaker antioxidant capacity than Phlegm(Wet)heat mutual card;2.The level of NO in Qi-Yin Deficiency type was significantly higher than that in Phlegm(Wet)heat-interaction type and control group.The higher level may have a causal relationship with SOD,MDA and GSH-Px,which may be related to type 2 diabetes mellitus with deficiency of both Qi-Yin Deficiency Patients longer duration related;3.Blood lipids,blood glucose and MDA may affect each other and play an important role in the pathogenesis of type 2 diabetes with deficiency of Qi-Yin Deficiency,Phlegm(Wet)heat mutual card,and may be a risk factor for the development of oxidative stress in type 2diabetes mellitus.