| Objective:Through(1H NMR)?enzyme linked immunosorbent assay(ELISA)?Western blotting(Western Blot WB)and microscopic observation of histopathology were performed by means of nuclear magnetic resonance spectroscopy(1H NMR).Effect of moxibustion and stomach meridian on serum,urine and gastric tissue in rats with chronic atrophic gastritis,and to explore the metabolic and related molecular mechanism and cell morphological changes of gastric mucosal injury in rats with chronic atrophic gastritis.Methods:48 SD rats were randomly divided into 4 groups:normal group,model control group,moxibustion model acupoint group,moxibustion model group,12 rats in each group.Each group was weighed and the rats were labeled with picric acid,and each rat was numbered accordingly.(0.1%)and deoxycholic acid solution(20mmol/L)were used to prepare the rat model of chronic atrophic gastritis[1],and then moxibustion was used in the other three groups except the normal group.Respectively,to stimulate the stomach group and non-acupoint group related parts.(1H NMR),enzyme linked immunosorbent assay(ELISA),Western blotting(Western Blot WB)were used to detect the serum,urine and gastric tissue of rats.The effects of moxibustion and stomach meridian on the serum,urine and gastric tissue of rats with chronic atrophic gastritis were observed under the observation of pathology and histology.The one-dimensional 1H NMR spectra were obtained and the multivariate statistical methods were used to identify each group And the morphological changes of gastric mucosal cells were investigated.The metabolic and related molecular mechanisms and morphological changes of gastric mucosal injury were induced by moxibustion.Results:The results of pathological biopsy showed that the normal tissue of the rats in the normal group was intact,the epithelium and glands were neat and uniform in size and shape.The cells were monolayer and had a cytoplasmic translocation/vacuole,glandular epithelium And the gland and the mucous membrane without dilation and blood stasis,mucosal myometrial hyperplasia,no pathological mitotic like;model group mucosa layer of the gland duct structure was disordered,duct morphology and size of the whole,Expansion of the duct,the size of the nucleus varies in the high magnification of the nucleus ratio increased,thick or loose reticular,nucleolus obvious;nucleus was rod-shaped or round,arranged uneven,visible mitotic;moxibustion The mucosal growth of the rats in the stomach meridian group was better than that in the model group,and the mucosal thickness and wrinkled wall were restored.The mucosa showed no obvious edema,atrophy,inflammation and neat gland,See the obvious atrophy,high magnification of the nucleus size is more uniform,neatly arranged,no pathological mitotic like;moxibustion non-acupoint group of mucosal cells significantly thinning atrophy,lamina propria and submucosal Can be seen more lymphocytes,plasma cells and mononuclear cell infiltration,mucosal muscle thickening,fibrous tissue hyperplasia,mucosal glandular atrophy,ductal morphology and size of the whole,the size of the nucleus,high magnification in the nuclear ratio Increased,thick stained,nucleolus obvious,arranged uneven,showing pathological mitotic like.The injury index of gastric mucosa was the lowest in the normal group,and the score of gastric mucosal injury index in the model group was significantly higher than that in the control group(P<0.05).The chronic atrophic(P<0.05).Compared with the model group,the gastric mucosal injury index of moxibustion group was significantly lower than that of moxibustion group(P<0.05),which indicated that moxibustion had significant effect on chronic atrophy(P<0.05),which indicated that moxibustion and stomach acupoints were more effective in the treatment of moxibustion than the acupoint group.Repair of Gastric Mucosa in Chronic Atrophic Gastritis Injury.The changes of blood flow in the gastric mucosa of rats in each group showed that the blood flow in the gastric mucosa of the model group was significantly decreased compared with the normal group.The gastric mucosal tissue of the moxibustion group Blood flow were significantly increased,of which moxibustion stomach meridian group,the most significant increase in gastric mucosal blood flow.Compared with the model group,the levels of myo-Inositol,Glycine and isoleucine in the serum of the moxibustion were significantly higher than those of the normal group.Moxibustion creatinine,lactate,acetate,creatine,suine,glycine,choline,sugar beet The levels of metabolites such as betaine,succinate,and formate were also restored.The expression of Glycine,Inositol,Aspartic acid,Mannitol and Creatine Kinase in the stomach tissue of the moxibustion were increased,2-Hydroxypropanoic acid,L-Glutamic Acid Hydrochloride,Ethyl 3-Hydroxybutyrate,DL-Methionine,Acetate,Glucose(Glucose),Isoleucine(Isoleucine)expression decreased,more tend to normal group of metabolic profile levels.Conclusion:The changes of molecular and pathological changes of urine and gastric tissue in rats with chronic atrophic gastritis were detected by means of related molecular mechanisms and pathology,metabolic histology of nuclear magnetic resonance and other methods.Moxibustion and stomach meridian intervention in chronic atrophic gastritis model of metabolic changes in the law,further reveals the chronic atrophic gastritis injury and repair of the metabolic mechanism and foot yangming and stomach-related scientific connotation,from the perspective of material metabolism from the perspective of Ai Moxibustion of the meridian can regulate the metabolic pattern of chronic atrophic gastritis and its related molecular mechanism.It shows that moxibustion and stomach meridian have definite therapeutic effect on chronic atrophic gastritis,and the curative effect is closely related to the accuracy of acupoints,and also indirectly prove the existence of acupoints. |
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