The Effect Of Huazhuo Jiedu Ruanjian Granules On Expression Of IL-6 And STAT3 In H22 Hepatic Cancer Mice

Objective: To explore the mechanism of Huazhuo Jiedu Ruanjian Granules treat liver cancer,observe the effect of Huazhuo Jiedu Ruanjian Granules inhibiting H22 transplanted hepatocelluar carcinoma growth and the expressions of IL-6 and STAT 3 in tumor tissues.Thus,it provides some experimental basis for clinical application.Methods: The cleaning grade of 40 healthy Kumming Mice,were randomly divided into groups according to weight,8 mice in the blank group(N),the remaining 32 mice established the H22 hepatic cancer mice model by hypodermic inoculation in the right axilla.After the model is successful,on the principle of randomization,32 of tumor-bearing mice were subdivided into four groups: model group(M),Huazhuo Jiedu Ruanjian Granules group(Z),5-Fu group(X),and Huazhuo Jiedu Ruanjian Granules combination with 5-Fu group(C),8 mice in each group.Each group was given the coreesponding drug intervention 7days in a row.After 12 h of the last dose,the mice were killed by the bone marrow dislocated method.Then,open abdominal cavity and blunt dissection tumor,which were weighed respectively.The last,we caculated the tumor inhibiting rates,and detected the espression level of IL-6 and STAT 3 of tumor tissues which fixed in 10% neutral formaldehyde with the immunohistochemical method,the remaining fresh tissue was put into the frozen storage tube,and the expression level of IL-6 and STAT3 in tumor tissues was detected by Western Blot method.Results:1.The general condition of the miceCompared with the blank group of mice,the tumor-burdened group mice showed different levels of mental distress,decreased activity,delayed response,poor skin luster,and decreased feeding,among which the mice inthe model group showed the most obvious performance.Among the treatment groups,the mental state,activity level and feeding of the C group were better than the other treatment groups.The mental status,activity level and feeding of Z group were better than the X group.2.The comparison of the inhibition rate of the tumor among the groupsAmong the treatment groups,the C group inhibitiory rate was highest,the second was the X group,and the third was the Z group.Compared the average tumor weight of the tumor-burdened groups,the C group was the lowest,which was followed by the X group,the Z group,and the model group.As for the average tumor weight,the three treatment group were less than the model group,the difference significant(P<0.05);The C group average tumor weight was less than the X group significantly(P<0.05);the last,the Z group and the X group,have no difference(P>0.05).3.The comparison of the IL-6 and STAT3 expression of tumor-tissue in different tumor-burdened groups3.1 Comparison of IL-6 expression levels in tumor tissuesThe expression level of positive expression and protein of IL-6 in the tumor tissues of the treatment groups were lower than that in the model group,and the difference was statistically significant(P<0.05).Among the treatment groups,the decrease of the the expression level of positive expression and protein of IL-6 of the C group was the most obvious,and there was a statistical difference between the Z group and the X group(P<0.05).There was no significant difference between the Z group and the X group(P>0.05).3.2 Comparison of STAT3 expression levels in tumor tissuesThe expression level of positive expression and protein of STAT3 in the tumor tissues of the treatment groups were lower than that in the model group,and the difference was statistically significant(P<0.05).Among the treatment groups,the decrease of the C group was the most obvious,and there was a statistical difference between the Z group and the X group(P<0.05).There was no significant difference between the Z group and the X group(P>0.05).Conclusions:1.Huazhuo Jiedu Ruanjian Granules can effectively inhibit tumor growth in tumor mice,and improve the general condition of the H22tumor-burdened mice,reduce the tumor weight and increase the inhibitory rate.2.Huazhuo Jiedu Ruanjian Granules can improve the inflammatory microenvironment of tumor and reduce the level of IL-6,further inhibit the expression of STAT 3 in the tumor,so as to play the role of tumor growth in tumor-resistant mice.