Effect Of Qingre Jiedu Huazhuo Tablet On TPA/PAI-1 In Heart And Aorta Of Rats With Intestinal Endotoxemia In Liver Disease

ObjectiveA rat model of intestinal endotoxemia?Intestinal endotoxemia,IETM?was established by high fat and alcohol diet.To observe the expression of t PA and PAI-1in myocardium and aorta tissue of QingReJieDuHuaZhuoPian,to study the protection and mechanism of QingRe JieDuHuaZhuoPian on myocardium and aorta.Methods70 SD rats were fed for a period of 7 days,and then were randomly divided into two groups:blank control group?n=10?and model control group?n=60?.The model group was fed with high fat diet,and52 degrees of white liquor to make the model.The blank control group was fed with ordinary feed and distilled water.The dosage of 10ml/kg was 2 mg/kg,which lasted for about 3 months,and 2 times a week to adjust liquor and distilled water dosage.3 months later,the detection of blood endotoxin,myocardial and aortic pathological sections were to determine the success of the model.The successful model rats were randomly divided into two groups:model control group,Qingre Jiedu tablet group,10 rats in each group.QingRe JieDuHuaZhuoPiangroupweregavagedQingReJieDuHuaZhuoPian suspension by(10g/kg*d^-1),the model control group and blank control group was given equal dosage of distilled water,2 times/day?9am and 16pm?,and all rats were fed normal diet for 1 months.After 1 months of treatment,fasting for about 12hours,the animals were sacrificed and the abdominal aorta was collected and separated by centrifuge,and to detecte total cholesterol?TCHO?,triglyceride?TG?,high density lipoprotein?HDLC?,low density lipoprotein?LDLC?,alanine aminotransferase?ALT?,aspartate aminotransferase?AST?,direct bilirubin?DBIL?,indirect bilirubin?IBIL?,with automatic biochemical.Plasma endotoxin?LPS?was measured by dynamic turbidimetric assay;The expression of plasminogen activator?tPA?and plasminogen activator inhibitor?PAI?protein of myocardial and aortic tissuewas detected by immunohistochemistry.Results1.The pathological changes of myocardial tissue in ratsIn the blank control group,the myocardial tissue structure was normal,no cell degeneration,necrosis and inflammatory cell infiltration were observed;In the model control group,rats were accompanied by myocardial hypertrophy,arrangement disorder,inflammatory cell infiltration and edema;The arrangement and structure of myocardial cells in QingRe JieDuHuaZhuoPian group were significantly improved,and the inflammatory cell infiltration was not obvious.2.Pathological changes of aorta in ratsIn the blank control group,the endothelial cells of aorta were intact,the intima of artery wall was smooth and non proliferation,and no inflammatory cells were found;In the model control group,the intima of aorta wall became thicker and thicker,and endothelial cells proliferated,and the inflammatory cell infiltration was not obvious.The structural integrity of the aortic endothelial cells and the arterial wall was significantlyimproved,andafewinflammatorycellswerefoundin QingRe JieDuHuaZhuoPian group.3.Comparison of plasma endotoxin in ratsCompared with blank control group,the plasma endotoxin in model control group was significantly different?P<0.01?;After treatment,the plasma endotoxin level of QingReJieDuHuaZhuoPian group was significantly lower than that of the model group?P<0.01?.4.Comparison of ALT,AST,DBIL and IBIL in each groupCompared with the blank control group,the serum ALT and AST of the model group were significantly higher?P<0.01?;Compared with the model group,the serum ALT,AST,DBIL and IBIL were significantly decreased?P<0.01,P<0.05?.5.Comparison of TCHO,TG,HDL and LDLC in serum of rats in each groupCompared with the blank control group,the serum TCHO,HDLC and LDLC were significantly higher in the model group?P<0.01?;Compared with the model group,theTCHOandLDLCweresignificantlydecreasedin QingRe JieDuHuaZhuoPian group?P<0.01?.6.Compare the expression of tPA and PAI-1 in myocardium and aorta of rats in each groupCompared with the normal control group,the expression of tPA and PAI-1 in myocardium and aorta of model group were significantly different?P<0.01?;After treatment,compared with the model group,the expression of tPA in the myocardial tissue of QingReJieDuHuaZhuoPian group was increased?P<0.05?,and the expression of PAI-1 was significantly reduced?P<0.01?;The expression of tPA in aorta tissue was significantly increased?P<0.01?,and the expression of PAI-1 was decreased?P<0.05?.7.Correlation analysisThere was a positive correlation between plasma endotoxin and serum ALT and AST;There was a negative correlation between LPS and tPA protein?r=-0.628,P<0.01?;There was a positive correlation between LPS and PAI-1 protein?r=595,P<0.01?;There was a negative correlation between LPS and aortic tPA protein?r=-0.572,P<0.05?.There was a positive correlation between LPS and PAI-1 protein?r=689,P<0.01?;There was a negative correlation between tPA protein and serum AST,and PAI-1 protein was positively correlated.Conclusion1.In the aspect of histomorphology,QingReJieDuHuaZhuoPian can improve the structure of myocardium and aorta in rats with IETM,and reduce the inflammatory changes.2.QingRe JieDuHuaZhuoPian can improve the liver function and lipid metabolism,reduce the level of plasma endotoxin,regulate the imbalance of intestinal flora and reduce the systemic inflammatory response.3.T The mechanism of QingReJieDuHuaZhuoPian to treat the IETM in rats is that QingReJieDuHuaZhuoPianQingReJieDuHuaZhuoPian can reduce the level of LPS,and coordinate the dynamic balance between fibrinolytic system tPA/PAI-1.