Effect Of Huazhuo Jiedu Yukuijian On MMP-2 And MIF In Rats With Ulcerative Colitis

Objective:In this study,the effects of Huazhuo Jiedu Yukuijian on the general state,pathological changes of colonic mucosa,disease activity index(DAI)score and expression of matrix metalloproteinase-2(MMP-2)and macrophage migration inhibitory factor(MIF)in rats with ulcerative colitis(UC)were studied.To explore the mechanism of Huazhuo Jiedu Yukuijian in the treatment of UC,and to provide experimental basis for clinical application.Methods:Sixty healthy Wistar rats were fed adaptively for one week.They were randomly divided into six groups with 10 rats in each group:control group(D),model group(M),western medicine mesalazine group(MS),high dose group(Z1),medium dose group(Z2)and low dose group(Z3).Except the control group,the other five groups were established UC rat model by TNBS/ethanol combination method.After the model was successful established,the rats were given normal saline,mesalazine,resolving turbidity and detoxification,clearing away toxin and clotting high,medium and low doses of gavage intervention for 2 weeks.The general state of the rats in each group was observed and recorded,and make a DAI score.After the treatment,the serum MMP-2 levels were detected by ELISA,and the expression condition of MIF in intestinal mucosa was detected by immunohistochemistry.Results:1.General condition and histopathological changesDuring the whole experimental period,D rats were in good condition.By the end of the treatment,the general state of UC rats and the general morphology and tissue damage of colon mucosa were improved,especially in Z1 group,the results of Z2 and MS groups were similar.2.DAI score results and Indicator detectionCompared with D rats,DAI score of M rats was significantly higher(P<0.05),serum MMP-2 and intestinal mucosa MIF content were significantly higher(P<0.05);compared with M rats,DAI score of each treatment group was significantly lower(P<0.05),MMP-2 and MIF content were significantly lower(P<0.05);Compared with MS,Z2 and Z3,DAI score of Z1 rats was lower(P<0.05),MMP-2 and MIF content decreased significantly(P<0.05);compared with Z3,DAI score of MS and Z2 was lower(P<0.05),MMP-2 and MIF content decreased significantly(P<0.05);The DAI score of MS and Z2 had no significant difference(P>0.05),MMP-2 and MIF content had no significant difference(P>0.05).Conclusions:Huazhuo Jiedu Yukuijian can alleviate the inflammatory reaction of UC rats and promote the healing of ulcer surface of intestinal mucosa.Its mechanism may be achieved inhibiting the expression of MMP-2and MIF.